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Conditions of optimal recombinant Human CA9/Carbonic Anhydrase IX Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP005525: Human CA9/Carbonic Anhydrase IX Protein with the C-His-Avi Tag
Source: The recombinant Human CA9/Carbonic Anhydrase IX Protein, (amino acids Gln38-Asp414) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CA9/Carbonic Anhydrase IX protein with the His-Avi tag at the C-terminus has a predicted MW of 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CA9 protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-CA9 Antibody protein with the C-hFc tag with the EC50 of 8.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CA9/Carbonic Anhydrase IX Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Human CA9/Carbonic Anhydrase IX Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following Mouse CA Protein :
Cynomolgus CA9 Protein
Human CA9 Protein
Mouse CA9 Protein
CA3 Protein
Syd Labs provides the following CA Enzyme :
CA1 Enzyme
CA4 Enzyme
CA5B Enzyme
CA7 Enzyme
CA8 Enzyme
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