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Conditions of optimal recombinant Human Activin RIIB/ACVR2B Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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BP003421: Human Activin RIIB/ACVR2B Protein with the C-His Tag
Source: The recombinant Human Activin RIIB/ACVR2B Protein, (amino acids Ser19-Thr137) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human Activin RIIB/ACVR2B protein with the His tag at the C-terminus has a predicted MW of 14.75 kDa. Due to glycosylation, the protein migrates to 25-45 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Activin A protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human Activin RIIB protein with the C-His tag with the EC50 of 21.4 ng/ml determined by ELISA (QC Test). The affinity constant of 27.92 pM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Activin RIIB/ACVR2B Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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