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Conditions of optimal GST? antibody performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Background
Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11 .Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.
PA000537-PA1040: GSTπ Polyclonal Antibody
Rabbit Polyclonal Antibody.
Application: Western blot: At 1-2 ug/ml with the appropriate system to detect GSTpi in cells and tissues; Immunohistochemistry(P): At 1-2 ug/ml to detect GSTpi in formalin fixed and paraffin embedded tissues. Boiling the sections is required; Immunocytochemistry: At 0.5-1 ug/ml to detect GSTpi in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.
Reactivity: Human, Mouse.
Immunogen: A peptide mapping at the C-terminus of GSTpi of human origin, different from the related mouse sequence by two sequence.
urification: Immunogen affinity purified.
Isotype: Rabbit IgG.
Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
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