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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal FVIII Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Background
Coagulation factor VIII (FVIII) is an essential blood clotting factor also known as anti-hemophilic factor (AHF). In humans, FVIII is encoded by the F8 gene(1-2). Defects in this gene results in hemophilia A, a well known recessive X-linked coagulation disorder(3). Factor VIII participates in blood coagulation; it is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids forms a complex that converts factor X to the activated form Xa. FVIII is processed from a single-chain polypeptide having the domain structure A 1 -A2-B-A3-C 1 -C2. The heavy chain is composed of domains Al-A2-B while the light chain is composed of domains A3-Cl-C2. The B domain is extensively glycosylated on asparagine, serine, and threonine residues(4). Initial studies showed that deletion of the B domain resulted in a functional molecule as tested by in vitro assays and was expressed more efficiently compared with wildtype FVIII(5).
References:
1. Toole JJ, Knopf JL, et al. (1984). Nature 312 (5992): 342?.
2. Truett MA, Blacher R, et al. (1985). DNA 4 (5): 333?9.
3. Antonarakis SE (1995). Haemost. 74 (1): 322?.
4. Vehar GA, Keyt B, et al. (1984). Nature 312:337.
5. Toole JJ, Pittman DD, et al. (1986) Proc Natl Acad Sci USA 835939.
BP000037-GD12: Recombinant Human FVIII Protein
Source: 293 cell-derived.
Ala20-Gsn764, Pro1659-Ly2351, Accession# NP_000123
N-terminal sequence analysis: Ala
Predicted molecular mass: 170 kDa
SDS-PAGE: 96 kDa, reducing conditions
The potency per mg of recombinant human FVIII protein was tested and found to be 11,000 Units/mg.
Endotoxin Level: <1.0 EU per 1 ug of the protein by the LAL method.
Purity > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Formulation: Lyophilized from a 0.2 um filtered solution of 27 ug/ml sodium chloride, 3.0 ug/ml sucrose, 1.5 ug/ml L-histidine, 0.25 ug/ml calcium chloride dihydrate, and 0.1 ug/ml polysorbate 80, pH 6.8.
Reconstitution: Reconstitute at 1 ug/ml in sterile H2O.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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