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Conditions of optimal FGF Acidic Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
FGF acidic is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors.
BP000181-C043: Recombinant Mouse Fibroblast Growth Factor Acidic (FGF Acidic) Protein
Source: E. coli-derived.
N-terminal sequence analysis: Phe-Asn-Leu-Pro-Leu.
The ED50 as determined by the dose-dependant stimulation of thymidine uptake by 3T3 cells in the presence of heparin was found to be less than 0.5 ng/ml, corresponding to a Specific Activity of 2.0 x 106 IU/mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM TrisHCl, 500 um NaCl, pH6.6.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other FGF Acidic Proteins:
Recombinant Human FGF acidic Protein
Recombinant Rat FGF acidic
Recombinant Mouse FGF acidic, His Tag
Recombinant Mouse FGF Acidic Protein
Recombinant Human FGF Acidic Protein, Sf9
Recombinant Human FGF Acidic Protein
Bovine FGF Acidic Protein
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