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Conditions of optimal FCGR2A Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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Introduction
Receptors for the Fc region of IgG (Fc Gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human Fc Gamma Rs are divided into three classes designated Fc Gamma RI (CD64), Fc Gamma RII (CD32), and Fc Gamma RIII (CD16), which generate multiple isoforms, are recognized. The activating type receptor either has or associates nonovalently with an accessory subunit that has an immunoreceptor tyrosine based activation motif (ITAM) in its cytoplasmic domain. Fc Gamma RI binds IgG with high affinity and functions during early immune responses, whereas Fc Gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human Fc Gamma RII (A, B and C) and one for mouse (Fc Gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc Gamma RII A and C) or ITIM motifs (Fc Gamma RIIB) in their cytoplasmic domains, have been identified.Human CD32, also known as Low affinity immunoglobulin gamma Fc region receptor II-a (IgG Fc receptor II-a), Fc Gamma RII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, Fc R Gamma, CD32a (Fc Gamma RII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as Fc Gamma RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
BP001443-C318: Recombinant Human Fc Gamma RIIA (FCGR2A) Protein
Source: HEK 293-derived.
Recombinant Human Fc Gamma RIIA (FCGR2A) Protein is expressed with sequence (Gln34-Val219) of Human FCGR2A fused with a polyhistidine tag at the C-terminus.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PBS, 150 mM NaCl, pH7.4.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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