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EK000074-EK0735: Human CXCL10/IP-10 ELISA Kit
Range 31.2pg/ml-2000pg/ml
Sensitivity < 1 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human CXCL10 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts.1 CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.2, 3 It is a potent inhibitor of angiogenesis in vivo.4 The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines.5 It is a RAS target gene and is overexpressed in the majority of colorectal cancers.6 This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.7
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Luster et al. Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins. Nature 315: 672-676, 1985.
2. Dufour et al. IFN-gamma-inducible protein 10 (IP-10; CXCL10) -deficient mice reveal a role for IP-10 in effector T cell generation and trafficking. J. Immun. 168:, 2002.
3. Angiolillo et al. Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. J. Exp. Med. 182: 155-162, 1995.
4. Angiolillo, A. L.; Sgadari, C.; Taub, D. D.; Liao, F.; Farber, J. M.; Maheshwari, S.; Kleinman, H. K.; Reaman, G. H.; Tosato, G. : Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. J. Exp. Med. 182: 155-162, 1995.
5. O'Donovan et al. Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet. Cell Genet. 84: 39-42, 1999.
6. Zhang, R.; Zhang, H.; Zhu, W.; Pardee, A. B.; Coffey, R. J., Jr.; Liang, P. : Mob-1, a Ras target gene, is overexpressed in colorectal cancer. Oncogene 14: 1607-1610, 1997.
7. Booth et al. The CXCR3 binding chemokine IP-10/CXCL10: structure and receptor interactions. Biochemistry 41:, 2002.
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