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Biotinylated Human TNF alpha Protein

APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2

Catalog No. Product Name Size List Price (US$) Quantity
BP005287 Biotinylated Human TNF alpha Protein with the C-His-Avi Tag 100 ug 525.00
BP005287 Biotinylated Human TNF alpha Protein with the C-His-Avi Tag 500 ug 1750.00
Description

BP005287: Biotinylated Human TNF alpha Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human TNF alpha Protein, (amino acids Val77-Leu233) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human TNF alpha protein with the His-Avi tag at the C-terminus has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-24 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human TNF R2 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human TNF alpha protein with the C-His tag with the EC50 of 5.1 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human TNF alpha Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Biotinylated Human TNF alpha Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

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