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Conditions of optimal CRISP2 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Cysteine-Rich Secretory Protein 2 (CRISP2) is a member of the CRISP subdivision of the CAP superfamily, which is defined by a CAP signature motif common to the entire superfamily, and has been localized to both the sperm acrosome and tail. Within mammals, four CRISPs have been defined: CRISP1 (acidic epididymal glycoprotein [AEG], proteins D and E), CRISP2 (TPX1), CRISP3, and CRISP4. All are strongly expressed within the male reproductive tract, suggesting key roles in male fertility. CRISP2 may regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation.
BP001677-C596: Recombinant Human Cysteine-Rich Secretory Protein 2 (CRISP2) Protein
Source: HEK 293-derived.
Recombinant Human Cysteine-Rich Secretory Protein 2 (CRISP2) Protein is expressed with sequence (Lys22-Tyr243) of Human CRISP2 fused with a polyhistidine tag at the C-terminus.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, 0.5% CHAPS, pH 7.4.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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