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Conditions of optimal recombinant Human Transferrin Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
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BP004423: Human Transferrin Protein with the C-His Tag
Source: The recombinant Human Transferrin Protein, (amino acids Val20-Pro698) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human Transferrin protein with the His tag at the C-terminus has a predicted MW of 76.3 kDa. Due to glycosylation, the protein migrates to 78-82 kDa based on Bis-Tris PAGE result.
Activity: The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Transferrin Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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