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Conditions of optimal recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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BP005710: Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein with the C-His-Avi Tag
Source: The recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein, (amino acids Gly17-Gln208(F176)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) protein with the His-Avi tag at the C-terminus has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Anti-Fc gamma RIIIA Antibody protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Human Fc gamma RIIIA (F176) protein with the C-His-Avi tag with the EC50 of 35.7 ng/ml determined by ELISA (QC Test). The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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