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EK000024-EK0312: Rat BMP-2 ELISA Kit
Range 62.5pg/ml-4000pg/ml
Sensitivity < 2pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of rat BMP-2 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-?) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Gopal Rao, V. V. N.; Loffler, C.; Wozney, J. M.; Hansmann, I. The gene for bone morphogenetic protein 2A (BMP2A) is localized to human chromosome 20p12 by radioactive and nonradioactive in situ hybridization. Hum. Genet. 90: 299-302, 1992.
2. Hallahan, A. R.; Pritchard, J. I.; Chandraratna, R. A. S.; Ellenbogen, R. G.; Geyer, J. R.; Overland, R. P.; Strand, A. D.; Tapscott, S. J.; Olson, J. M. BMP-2 mediates retinoid-induced apoptosis in medulloblastoma cells through a paracrine effect. Nature Med. 9: 1033-1038, 2003.
3. Cheng, H.; Jiang, W.; Phillips, F. M.; Haydon, R. C.; Peng, Y.; Zhou, L.; Luu, H. H.; An, N.; Breyer, B.; Vanichakarn, P.; Szatkowski, J. P.; Park, J. Y.; He, T.-C. Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J. Bone Joint Surg. 85-A: 1544-1552, 2003.
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