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Conditions of optimal recombinant Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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BP003855: Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein with the C-His Tag
Source: The recombinant Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein, (amino acids Leu23-Asp257) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human TNFR2/CD120b/TNFRSF1B protein with the His tag at the C-terminus has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Biotinylated Human TNFR2 protein with the C-His tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Anti-TNFR2 Antibody protein with the C-hFc tag with the EC50 of 9.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human TNFR2/CD120b/TNFRSF1B Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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