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Biotinylated Human TLR3 Protein

TLR3; CD283; IIAE2

Catalog No. Product Name Size List Price (US$) Quantity
BP004148 Biotinylated Human TLR3 Protein with the C-His Tag 100 ug 525.00
BP004148 Biotinylated Human TLR3 Protein with the C-His Tag 500 ug 1750.00
BP003081 Biotinylated Human TLR3 Protein with the C-His-Avi Tag 100 ug 525.00
BP003081 Biotinylated Human TLR3 Protein with the C-His-Avi Tag 500 ug 1750.00
Description

BP004148: Biotinylated Human TLR3 Protein with the C-His Tag

Source: The recombinant Biotinylated Human TLR3 Protein, (amino acids Ser23-Glu703) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human TLR3 protein with the His tag at the C-terminus has a predicted MW of 78.7 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Biotinylated Human TLR3 protein with the C-His tag at 0.5 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Anti-TLR3 Antibody protein with the C-hFc tag with the EC50 of 6.6 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human TLR3 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP003081: Biotinylated Human TLR3 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human TLR3 Protein, (amino acids Ser23-Glu703) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human TLR3 protein with the His-Avi tag at the C-terminus has a predicted MW of 80.25 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Biotinylated Human TLR3 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/Well) on streptavidin (5 ug/ml) precoated plate. Dose response curve for the recombinant Anti-TLR3 Antibody protein with the C-hFc tag with the EC50 of 2.3 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human TLR3 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Biotinylated Human TLR3 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

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