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Home > Antibodies > A-Z Primary Antibodies > Biotinylated Human CD40 Ligand Protein

Biotinylated Human CD40 Ligand Protein

CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand

Catalog No. Product Name Size List Price (US$) Quantity
BP005686 Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein with the N-His-Flag Tag 100UG 525.00
BP005686 Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein with the N-His-Flag Tag 500UG 1750.00
Description

BP005686: Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein with the N-His-Flag Tag

Source: The recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein, (amino acids Met113-Leu261) fused with a His-Flag tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer protein with the His-Flag tag at the N-terminus has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Biotinylated Human CD40 Ligand Trimer protein with the N-His-Flag tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for the recombinant Human CD40 protein with the N-hFc tag with the EC50 of 0.70 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

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