Anti-Mouse OX40 Monoclonal Antibodies for Flow Cytometry

PA007503.r1: Recombinant Anti-mouse OX40 Monoclonal Antibody (Clone: OX86), Rat IgG1 Kappa

Recombinant Rat IgG1 Monoclonal Antibody. 
Clone: OX86.
Isotype:  Rat IgG1 Kappa.
Source: The anti-mouse OX40 monoclonal antibody (clone: OX86) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant OX86 antibody specifically binds to mouse OX40 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

PA007503.h1Fs: Recombinant Anti-mouse OX40 Monoclonal Antibody (Clone: OX86), Human IgG1 Fc Silent Kappa

Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation. 
Clone: OX86.
Isotype: Human IgG1 kappa.
Source: The anti-mouse OX40 monoclonal antibody (clone: OX86) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant OX86 antibody specifically binds to mouse OX40 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.

Shipping: The OX86 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Background
The rat anti-mouse OX40 / CD134 monoclonal antibody OX86 (rat IgG1 kappa) reacts with the mouse OX40 protein (CD134), a costimulatory molecule that belongs to the tumor necrosis factor receptor (TNFR) superfamily. OX40 agonist-based combinations are emerging as promising candidates for novel cancer immunotherapy. The OX86 antibody is recommended for detection of mouse OX40 cell surface antigen by IF and FCM available conjugated to either phycoerythrin or FITC for IIHC (P) and FCM.
Our recombinant OX86 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse OX40 monoclonal antibody (hybridoma clone name or number: OX86).

Syd Labs provides the following in vivo grade recombinant anti-mouse OX40 monoclonal antibodies:
Recombinant Anti-mouse OX40 monoclonal antibody (Clone: OX86)

References of anti-mouse OX40 antibody for Flow Cytometry (Clone OX86):

Anti-OX40 Antibody Limits Antitumor T Cell Expansion but Protects from Tumor Relapse
Redjimi I, et al. Cancer Immunol Res. 2020 Apr;8(4):533-545. doi: 10.1158/2326-6066.CIR-19-0805. PMID: 32024639
The OX86 antibody, a monoclonal antibody targeting OX40, was used to investigate its role in limiting antitumor T cell expansion while protecting against tumor relapse in preclinical mouse models. Fluorescently conjugated OX86 antibodies were employed in flow cytometry to assess CD45, CD4, CD8, and OX40 expression in tumor single-cell suspensions, analyzed on a BD LSRFortessa flow cytometer. The study observed a significant increase in CD8+ T cell frequency in the tumor microenvironment post-treatment. Fc-dependent activity of anti-OX40 antibodies was evaluated using FcγR-deficient mice, and tumor relapse was monitored over 60 days following treatment cessation.
Tags: activity of OX86 antibody; function of OX86 antibody

OX40 Costimulation Inhibits Foxp3 Expression and Treg Induction via BATF3-Dependent and Independent Mechanisms
Xiao X, et al. Cell Mol Immunol. 2012 Sep;9(5):386-392. doi: 10.1038/cmi.2012.23. PMID: 22745387
The OX86 monoclonal antibody was utilized to detect OX40 expression on CD4+ T cells through flow cytometry, alongside anti-CD4 and anti-CD25 antibodies. The study demonstrated that OX40 ligation suppresses Foxp3 expression in naive CD4+ T cells, impacting Treg induction. BATF3-deficient mice were used to explore the molecular pathways involved, with flow cytometry data analyzed using FlowJo software to gate Treg populations. An in vitro suppression assay confirmed reduced Treg function following OX40 stimulation.
Tags: function of OX86 mAb; OX86 antibody

OX40 (CD134) Controls Memory T Helper 2 Cells That Drive Lung Inflammation
Salek-Ardakani S, et al. J Immunol. 2011 Apr 1;186(7):3768-3776. doi: 10.4049/jimmunol.1101266. PMID: 21335488
The OX86 monoclonal antibody was used in flow cytometry experiments to assess OX40 expression on memory Th2 cells, identified by CD44 and IL-4 expression, in a mouse model of allergic airway inflammation induced by OVA challenge. Flow cytometry analysis revealed a significant increase in OX40+ Th2 cells in the lungs, and anti-OX40 treatment was shown to reduce airway hyperresponsiveness in the asthma model.
Tags: OX86 antibody for animal model; OX86 antibody for cancer research

Costimulatory Molecule OX40L Is Critical for Both Th1 and Th2 Responses in Allergic Inflammation
Arestides RS, et al. Eur J Immunol. 2002 Oct;32(10):2874-2880. doi: 10.1002/1521-4141(2002010)32:10<2874::AID-IMMU2874>3.0.CO;2-4. PMID: 12370369
The OX86 antibody was used in flow cytometry to stain OX40 on CD4+ T cells in a mouse model of allergic inflammation induced by intraperitoneal sensitization with OVA. The study found that OX40L-deficient mice exhibited reduced Th2 cytokine production, and the role of OX40-OX40L interactions was confirmed using blocking antibodies. Splenocytes were cultured for 48 hours prior to flow cytometry analysis.
Tags: OX86 antibody for flow cytometry; OX86 antibody for mouse tumor model

Cutting Edge: OX40 Inhibits Cytokine Production by CD4+ T Cells
Gramaglia I, et al. J Immunol. 2000 Nov 15;165(10):5302-5306. doi: 10.4049/jimmunol.165.10.5302. PMID: 11086044
The OX86 monoclonal antibody was employed to detect OX40 expression on activated CD4+ T cells isolated from spleen and lymph nodes of BALB/c mice via flow cytometry. The study showed that OX40 ligation significantly reduced IL-2 secretion, with cytokine production measured through intracellular staining for IFN-γ and IL-4. Experiments were conducted in triplicate to ensure reproducibility.
Tags: OX86 mAb for flow cytometry; OX86 monoclonal antibody

For more references about anti-mouse OX40 antibody for Flow Cytometry (Clone OX86), please contact our scientific support team with message@sydlabs.com.