Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: MECA-367.
Isotype: Rat IgG2a Kappa.
Source: The anti-mouse MAdCAM-1 monoclonal antibody (clone: MECA-367) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: MECA-367) specifically binds to mouse MAdCAM-1.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MAdCAM-1 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MAdCAM-1 monoclonal antibody of clone MECA-367 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Genomic Modifiers of Natural Killer Cells, Immune Responsiveness and Lymphoid Tissue Remodeling Together Increase Host Resistance to Viral Infection.
Gillespie, A. L., et al. PLoS Pathog. 2016 Feb 4;12(2):e1005419. doi: 10.1371/journal.ppat.1005419. PMID: 26845690
Briefly, frozen sections (6 μm) were fixed with a 1:1 mix of acetone/ethanol(100%) and stained with Abs against B220 (RA3-632, BD Bioscience), CD3 (145-2C11, Ebiosciences), MAdCAM-1 (MECA-367, BioXcell), CD209b/SIGN-R1 (eBio22D1, Ebiosciences), NKp46 (polyclonal, R&D systems), and CD169 (MOMA-1, AbDSerotec). ...As the extent of viral control differs in MA/My and Tg1 spleens (Fig 4), we considered that apparent differences in splenic SLO integrity could be due to the variance in viral titers. ...Abs and isotype controls for flow cytometry were purchased from BD Biosciences, Biolegend, and R&D Systems. ...Spleen and liver gDNA samples were prepared using a Gentraprep kit. ...We gratefully acknowledge the contribution of DartMouse to genome-wide genotyping performed in this study.
Tags: anti-mouse MAdCAM-1 MECA-367 in mouse tumor model; function of anti-mouse MAdCAM-1 MECA-367
Effector lymphocyte-induced lymph node-like vasculature enables naive T-cell entry into tumours and enhanced anti-tumour immunity.
Peske, J. D., et al. Nat Commun. 2015 May 13;6:8114. doi: 10.1038/ncomms8114. PMID: 25968334
In both tumour sites, PNAd+ vessels coexpressed MAdCAM-1 and VCAM-1 ...Naive OT-I cells were transferred into WT mice with i.p. B16-OVA tumours pre-treated with anti-MAdCAM-1 antibody or Rat IgG isotype control (h), or anti-PNAd antibody or Rat IgM isotype control (i). ...To determine whether infiltration was mediated by L-selectin binding to MAdCAM-1 or PNAd, we transferred OT-I cells into i.p. tumour-bearing mice pretreated to block luminally expressed molecules with injection of either anti-MAdCAM-1, anti-PNAd or isotype control. ...MAdCAM-1 blockade reduced infiltration into Peyer’s patches but had no effect on tumour infiltration. ...MAdCAM-1 did not contribute to naive T-cell infiltration, however, perhaps because it is not properly posttranslationally modified to serve as an L-selectin ligand.
Tags: bioactivity of anti-mouse MAdCAM-1 MECA-367; anti-mouse MAdCAM-1 MECA-367 antibody of low endotoxin
For more references about Anti-mouse MAdCAM-1 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
Copyright © 2009-2025 sydlabs.com. All rights reserved.
Use of this website means that you have read, understood, and accepted the Syd Labs Privacy Policy and Terms & Conditions.
Friend Link: Ushelf