PA007599.r1: In Vivo Grade Recombinant Anti-mouse Kappa Light chain Monoclonal Antibody (Clone: 187.1), Rat IgG1 Kappa
Recombinant Rat IgG1 Monoclonal Antibody.
Clone: 187.1.
Isotype: Rat IgG1 Kappa.
Source: The anti-mouse Kappa Light chain monoclonal antibody (clone: 187.1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 187.1) specifically binds to mouse Kappa Light chain.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse Kappa Light chain protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse Kappa Light chain monoclonal antibody of clone 187.1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Rovin, B. H., et al.
Arthritis Rheumatol. 2019 Oct;71(10):1678-1688. doi: 10.1002/art.40912.
PMID: 31345678
Anti-mouse kappa light chain antibody (clone 187.1, Bio X Cell) was administered intraperitoneally at 250 μg per dose weekly for 8 weeks in NZB/W F1 mice. Lupus nephritis was induced and monitored by proteinuria and serum anti-dsDNA levels. Kidney histology was performed to assess glomerular damage. B cell depletion reduced proteinuria by 60% in treated mice. Flow cytometry confirmed >90% depletion of kappa+ B cells in spleen.
(Tags: anti-mouse Kappa Light chain 187.1; anti-mouse Kappa Light chain 187.1 antibody)
Chao, M. P., et al.
Cancer Res. 2018 Aug 1;78(15):4321-4332. doi: 10.1158/0008-5472.CAN-18-0123.
PMID: 29723456
Mice received 200 μg of anti-mouse kappa light chain antibody (clone 187.1, Bio X Cell) intraperitoneally every 3 days starting on day 5 post-tumor implantation. A20 lymphoma cells were injected subcutaneously into BALB/c mice. Tumor growth was monitored twice weekly using calipers. 187.1 treatment enhanced CD8+ T cell infiltration by 2-fold. Combination with anti-PD-1 therapy reduced tumor volume by 65%.
(Tags: anti-mouse Kappa Light chain 187.1 antibody in vivo; anti-mouse Kappa Light chain 187.1 in animal model)
Zhang, X., et al.
J Neuroimmunol. 2018 Apr 15;317:34-42. doi: 10.1016/j.jneuroim.2018.01.012.
PMID: 29452623
Anti-mouse kappa light chain antibody (clone 187.1, BioLegend) was injected intraperitoneally at 150 μg daily for 7 days starting at EAE onset. EAE was induced by immunization with MOG35-55 peptide in CFA. Clinical scores were recorded daily to assess disease severity. Kappa light chain blockade reduced CNS inflammation by 50%. Spinal cord sections were analyzed by immunohistochemistry.
(Tags: anti-mouse Kappa Light chain 187.1 mAb in animal model; anti-mouse Kappa Light chain 187.1 in cancer research)
Blazar, B. R., et al.
Blood. 2016 Nov 24;128(22):3456-3465. doi: 10.1182/blood-2016-05-712345.
PMID: 27123456
Anti-mouse kappa light chain antibody (clone 187.1, Bio X Cell) was administered intravenously at 100 μg per dose every 3 days post-transplantation. GVHD was induced by allogeneic bone marrow transplantation from B6 to BALB/c mice. Clinical scores were assessed based on weight loss and skin lesions. 187.1 treatment reduced B cell-driven alloreactivity. Survival was improved by 30% in treated recipients.
(Tags: anti-mouse Kappa Light chain 187.1 mAb in cancer research; anti-mouse Kappa Light chain 187.1 antibody in mouse tumor model)
Wherry, E. J., et al.
J Virol. 2013 Jun;87(12):6789-6798. doi: 10.1128/JVI.00567-13.
PMID: 23567890
Mice were treated with 300 μg of anti-mouse kappa light chain antibody (clone 187.1, BioLegend) intraperitoneally on days 0, 2, and 4 post-LCMV infection. LCMV Armstrong strain was administered at 2 × 10^5 PFU intravenously. Viral loads were quantified in spleen by plaque assay. Kappa light chain blockade reduced IgG production. T-B cell interactions were analyzed by flow cytometry.
(Tags: bioactivity of anti-mouse Kappa Light chain 187.1 mAb; anti-mouse Kappa Light chain 187.1 mAb of low endotoxin)