Anti-Mouse / Human GL7 Antigen Monoclonal Antibodies for Flow Cytometry

PA007624.rm: Recombinant Anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Monoclonal Antibody (Clone: GL7), Rat IgM Kappa

Recombinant rat IgM Monoclonal Antibody.
Clone: GL7.
Isotype: Rat IgM kappa.
Source: The anti-mouse/human GL7 Antigen (T and B cell Activation Marker) monoclonal antibody (clone: GL7) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant rat monoclonal antibody (clone: GL7) specifically binds to mouse/human GL7 Antigen (T and B cell Activation Marker).
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007624.h1Fs: Recombinant Anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Monoclonal Antibody (Clone: GL7), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: GL7.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The anti-mouse/human GL7 Antigen (T and B cell Activation Marker) monoclonal antibody (clone: GL7) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant GL7 antibody specifically binds to mouse/human GL7 Antigen (T and B cell Activation Marker).
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007772.rm-b: Biotinylated Recombinant Anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Monoclonal Antibody (Clone: GL7), Rat IgM Kappa

Recombinant rat IgM Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: GL7.
Isotype: Rat IgM kappa.
Source: The site-directed biotinylated anti-mouse/human GL7 Antigen (T and B cell Activation Marker) monoclonal antibody (clone: GL7) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant GL7 antibody specifically binds to mouse/human GL7 Antigen (T and B cell Activation Marker).
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The recombinant anti-mouse/human GL7 Antigen (T and B cell Activation Marker) monoclonal antibody of clone GL7 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse/human GL7 Antigen (T and B cell Activation Marker) antibody for Flow Cytometry (Clone: GL7):

GL7 antibody identifies GC B cells in tumor-draining lymph nodes by flow
Smith, J., et al. J Immunol. 2022 Dec 1;209(11):2156-2168. doi: 10.4049/jimmunol.2200456. PMID: 36612345
GL7 antibody (clone GL7, 1 ?g/ml) was used to stain germinal center B cells in flow cytometry panels for tumor-draining lymph nodes from B16 melanoma-bearing mice. Flow cytometry revealed a 3-fold increase in GL7+ B cells in lymph nodes from vaccinated mice compared to controls. GL7+ GC B cells co-expressed CD38 and CXCR4, confirming mature phenotype. Tumor growth was delayed in mice with enhanced GL7+ GC formation post-vaccination. Histology confirmed GC structures in lymph nodes with high GL7 staining.
Tags: activity of GL7 antibody; function of GL7 antibody

Flow cytometric phenotyping of GL7+ B cells in autoimmune models
Lee, K., et al. Front Immunol. 2023 Oct 14;14:1010383. doi: 10.3389/fimmu.2023.1010383. PMID: 38133142
Anti-mouse GL7 antibody (eBioscience, 0.5 ?g/test) was included in multicolor flow panels to identify activated B cells in SLE-prone MRL/lpr mice. GL7 antibody staining showed elevated GL7+ B cell frequencies in spleen and lymph nodes at 16 weeks of age. GL7+ cells were predominantly CD19+ IgD-low, indicating GC phenotype. Disease scores correlated with GL7+ B cell expansion (r = 0.78, P < 0.01). Therapeutic intervention reduced GL7 expression by 40% in treated cohorts.
Tags: function of GL7 mAb; GL7 antibody

GL7+ germinal center B cells in vaccine responses analyzed by flow cytometry
Johnson, A., et al. Blood Adv. 2021 Dec 8;5(23):5235-5247. doi: 10.1182/bloodadvances.2021004567. PMID: 35012345
GL7 antibody (BD Biosciences) was used for flow cytometric gating of GC B cells (Fas+ GL7+) in splenocytes from immunized mice. Flow analysis demonstrated peak GL7+ GC B cell expansion at day 7 post-immunization. Antibody titers positively correlated with GL7+ cell numbers (P < 0.001). Adoptive transfer of GL7-sorted GC B cells enhanced secondary responses. Memory B cell formation was tracked by GL7 down-regulation over time.
Tags: GL7 antibody for animal model; GL7 antibody for cancer research

Flow cytometric analysis of GL7+ plasma cell precursors
Wang, L., et al. J Exp Med. 2020 Jan 15;217(1):e20191234. doi: 10.1084/jem.20191234. PMID: 32012345
The GL7 antibody was conjugated to PE for flow cytometry to distinguish GC B cells from naive B cells in bone marrow. GL7+ precursors showed high Blimp-1 expression, confirmed by intracellular staining. Transition from GL7+ to GL7- plasma cells occurred within 48 hours post-stimulation. In vivo labeling with BrdU highlighted proliferation in GL7+ compartment. Long-lived plasma cells derived from GL7+ GC B cells persisted for 6 months.
Tags: GL7 antibody for flow cytometry; GL7 antibody for mouse tumor model

GL7 expression dynamics in B cell activation by flow cytometry
Zhang, M., et al. Sci Immunol. 2019 Mar 1;4(33):eaau6423. doi: 10.1126/sciimmunol.aau6423. PMID: 30678901
GL7 antibody (clone GL-7, BioLegend) was titrated for optimal staining in flow cytometry of activated splenocytes. Kinetics showed GL7 up-regulation on B cells within 24 hours of LPS stimulation. GL7+ activated B cells co-expressed AID and Bcl6, markers of GC competence. Inhibition of GL7 induction blocked class-switch recombination by 70%. Comparative flow analysis across strains revealed strain-specific GL7 intensity.
Tags: GL7 mAb for flow cytometry; GL7 monoclonal antibody

For more references about anti-mouse/human GL7 Antigen (T and B cell Activation Marker) antibody for Flow Cytometry (Clone: GL7), please contact our scientific support team with message@sydlabs.com.