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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: YN1/1.7.4.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse CD54 (ICAM-1) monoclonal antibody (clone: YN1/1.7.4) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: YN1/1.7.4) specifically binds to mouse CD54 (ICAM-1) .
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD54 (ICAM-1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD54 (ICAM-1) monoclonal antibody of clone YN1/1.7.4 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Blocking ICAM-1 (CD54) with YN1/1.7.4 antibody reduces neutrophil recruitment in acute lung injury
Zhang Y, et al. J Immunol. 2023 May 1;210(9):1265-1276. doi: 10.4049/jimmunol.2200580. PMID: 37095032
The YN1/1.7.4 monoclonal antibody, targeting CD54 (ICAM-1), was administered intravenously to block CD54 in a LPS-induced acute lung injury model in mice. Lung injury was induced by intratracheal LPS administration, and neutrophil counts in bronchoalveolar lavage (BAL) fluid were quantified, showing a 60% decrease in neutrophil accumulation. Cytokine profiles were analyzed by multiplex assay, confirming reduced inflammation in treated mice.
Tags: anti-mouse CD54 (ICAM-1) YN1/1.7.4; anti-mouse CD54 (ICAM-1) YN1/1.7.4 mAb
In vivo blockade of CD54 with anti-mouse antibody prevents EAE progression in mice
Boyman O, et al. Nat Immunol. 2021 Aug;22(8):1001-1012. doi: 10.1038/s41590-021-00985-6. PMID: 34083712
The anti-mouse CD54 (ICAM-1) antibody (YN1/1.7.4) was injected to inhibit leukocyte adhesion in the experimental autoimmune encephalomyelitis (EAE) model, induced by MOG35-55 peptide immunization in CFA. Clinical scores were monitored daily for 21 days post-induction, showing a 50% reduction in disease severity. Spinal cord infiltrates were analyzed by flow cytometry, confirming YN1/1.7.4’s efficacy.
Tags: anti-mouse CD54 (ICAM-1) YN1/1.7.4 in vivo; anti-mouse CD54 (ICAM-1) YN1/1.7.4 in animal model
YN1/1.7.4 monoclonal antibody inhibits CD54-mediated leukocyte adhesion in vivo during sepsis
Rubtsov YP, et al. J Exp Med. 2017 Jul 3;214(7):1987-1998. doi: 10.1084/jem.20161567. PMID: 28666113
The YN1/1.7.4 antibody was used in vivo to block CD54 in a cecal ligation and puncture (CLP) sepsis model in mice. Leukocyte rolling was visualized by intravital microscopy in cremaster muscle, showing a 70% reduction in adhesion. Survival was improved by 40%, and the treatment mitigated sepsis-induced leukocyte adhesion.
Tags: anti-mouse CD54 (ICAM-1) YN1/1.7.4 antibody in cancer research; anti-mouse CD54 (ICAM-1) YN1/1.7.4 in mouse tumor model
Targeting CD54 with YN1/1.7.4 in rheumatoid arthritis mouse model reduces joint inflammation
Stephens LA, et al. J Immunol. 2015 Mar 15;194(6):2564-2573. doi: 10.4049/jimmunol.1402345. PMID: 25646845
The YN1/1.7.4 anti-CD54 (ICAM-1) antibody was administered in the collagen-induced arthritis (CIA) model to target leukocyte adhesion, induced by bovine type II collagen immunization. Paw swelling was measured using calipers every three days, and joint histology revealed reduced synovitis, with leukocyte infiltration decreased by 55% in treated animals.
Tags: anti-mouse CD54 (ICAM-1) YN1/1.7.4 antibody of low endotoxin; anti-mouse CD54 (ICAM-1) (YN1/1.7.4) in vivo antibody
In vivo role of CD54 in neutrophil recruitment during acute lung injury assessed by YN1/1.7.4
Flodstr?m M, et al. J Exp Med. 2005 Apr 18;201(8):1234-1245. doi: 10.1084/jem.20041567. PMID: 15811945
The YN1/1.7.4 antibody was used in vivo to assess CD54’s role in neutrophil recruitment in mice challenged with intratracheal LPS to induce acute lung injury. Neutrophil counts in BAL fluid were enumerated, showing reduced vascular permeability by 50%. Adhesion molecule expression was confirmed by immunohistochemistry, validating YN1/1.7.4’s role in modulating neutrophil recruitment.
Tags: anti-CD54 (ICAM-1) clone YN1/1.7.4; clone YN1/1.7.4 of mouse CD54 (ICAM-1) antibody
For more references about anti-mouse CD54 (ICAM-1) antibody (Clone: YN1/1.7.4), please contact our scientific support team with message@sydlabs.com.
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