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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: YN1/1.7.4.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse CD54 (ICAM-1) monoclonal antibody (clone: YN1/1.7.4) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: YN1/1.7.4) specifically binds to mouse CD54 (ICAM-1) .
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD54 (ICAM-1) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse CD54 (ICAM-1) monoclonal antibody of clone YN1/1.7.4 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
PLZF induces an intravascular surveillance program mediated by long-lived LFA-1-ICAM-1 interactions.
Thomas, S. Y., et al. J Exp Med. 2011 Jun 6;208(6):1179-88. doi: 10.1084/jem.20102630. PMID: 21624939
Injection of blocking antibodies against LFA-1 and ICAM-1 induced the rapid release of liver NKT cells into the peripheral blood, demonstrating the critical role of these integrins in the long-term intravascular residence of NKT cells. ...Blocking antibodies against mouse CD11a (LFA-1; M17/4), CD54 (ICAM-1; YNI.7.4), CD49d (VLA-4; PS/2), and CD106 (VCAM-1; M/K-2.7) and rat IgG2a Control (2A3) were purchased from BioXCell and injected iv 3 h before tissue harvest. ...Within 3 h, we observed a fivefold drop on average in the NKT cell numbers recovered from the liver. ...This massive drop was accompanied by a 10-fold increase of the frequency of NKT cell in the peripheral recirculating blood, which now exceeded that of lymphoid tissues. ...In contrast, blocking with anti?VLA-4 and anti?VCAM-1, which play a substantial role in the intraluminal crawling and transmigration of conventional effector T cells, had no effect.
Tags: anti-mouse CD54 (ICAM-1) YN1/1.7.4; anti-mouse CD54 (ICAM-1) YN1/1.7.4 mAb
Hapten application to the skin induces an inflammatory program directing hapten-primed effector CD8 T cell interaction with hapten-presenting endothelial cells.
Kish, D. D., et al. J Immunol. 2011 Feb 15;186(4):2117-26. doi: 10.4049/jimmunol.1002337. PMID: 21239709
Contact hypersensitivity is a CD8 T cell-mediated response to hapten sensitization and challenge of the skin. ...Effector CD8 T cell recruitment into the skin parenchyma to elicit the response to hapten challenge requires prior CXCL1/KC-directed neutrophil infiltration within 3?6 h after challenge and is dependent on IFN-? and IL-17 produced by the hapten-primed CD8 T cells. ...Mechanisms directing hapten-primed CD8 T cell localization and activation in the Ag challenge site to induce this early CXCL1 production in response to 2,4-dinitrofluorobenzene were investigated. ...Both TNF-? and IL-17, but not IFN-?, mRNA was detectable within 1 h of hapten challenge of sensitized mice and increased thereafter.These results indicate that hapten application to the skin of sensitized animals initiates an inflammatory response promoting hapten-primed CD8 T cell localization to the challenge site through TNF-??induced ICAM-1 expression and CD8 T cell activation to produce IFN-? and IL-17 through endothelial cell presentation of hapten.
Tags: anti-mouse CD54 (ICAM-1) YN1/1.7.4 in vivo; anti-mouse CD54 (ICAM-1) YN1/1.7.4 in animal model
Effects of ICAM-1 antisense oligonucleotide on the tubulointerstitium in mice with unilateral ureteral obstruction.
Cheng, Q. L., et al. Kidney Int. 2000 Jan;57(1):183-90. doi: 10.1046/j.1523-1755.2000.00825.x. PMID: 10620199
Four micrometer cryosections were incubated with 5 ?g/mL of rat antimouse ICAM-1 monoclonal antibody YN1/1.7.4, with 15 ?g/mL of Mac-1 antibody (Pharmingen, San Diego, CA, USA). ...The ICAM-1 expression on the TEC surface was determined by immunocytochemical staining. ICAM-1 monoclonal antibody YN1/1,7,4 (1 ?g/mL) was a gift from Dr. Matsuda (Juntendo University, Tokyo, Japan). ...Group A was treated with ICAM-1 ASON, which was injected intravenously at the dosage of 1 mg/kg/day every other day for one week. ...After being prehybridized, the blots were hybridized with mouse ICAM-1 cDNA probe labeled with [?-32P]dCTP using random primed DNA labeling kit (Boehringer Mannheim, Germany) at 65°C for 24 hours. Autoradiography was performed at -70°C using intensifying screens. ...To obtain information about the distribution of ICAM-1 ASON in mice, we injected the FITC-labeled ICAM-1 ASON via tail vein at 10 mg/kg into either normal mice or mice with UUO operation for 16 hours.
Tags: anti-mouse CD54 (ICAM-1) YN1/1.7.4 antibody in cancer research; anti-mouse CD54 (ICAM-1) YN1/1.7.4 in mouse tumor model
For more references about Anti-mouse CD54 (ICAM-1) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
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