Anti-Human Siglec-2/CD22 Monoclonal Antibodies for Flow Cytometry

PA007299: Non-conjugated Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)

Recombinant  Mouse IgG2a Monoclonal Antibody. 
Clone: NCI m971.
Isotype:  Mouse IgG2a kappa.
Source: The recombinant anti-human Siglec-2/CD22 monoclonal antibody (clone: NCI m971) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant NCI m971 antibody specifically binds to the human Siglec-2/CD22 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F). 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007300: Biotin Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)

Recombinant mouse IgG2a Monoclonal Antibody with site-directed biotin conjugation through the Avi tag.
Clone: NCI m971.
Isotype: Mouse IgG2a kappa.
Source: The biotin anti-mouse Siglec-2/CD22 monoclonal antibody (clone: NCI m971) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The biotin recombinant NCI m971 antibody specifically binds to the human Siglec-2/CD22 protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin. 
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The NCI m971 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.

Syd Labs provides the following recombinant anti-human Siglec-2/CD22 monoclonal antibodies for flow cytometry:

Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
Biotin Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
FITC Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
APC Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PE Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
Pacific Blue Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PerCP Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PE/Cy5 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PE/Cy7 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 488 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 549 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 594 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 647 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 700 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
APC/Cy7 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PerCP/Cy5.5 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)

Background:

The recombinant NCI m971 antibody binds to the human Siglec-2/CD22 protein, a sugar binding transmembrane protein, which specifically binds sialic acid with an immunoglobulin (Ig) domain located at its N-terminus. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice. In mice, it has been shown that CD22 blockade restores homeostatic microglial phagocytosis in aging brains.

References of anti-human Siglec-2/CD22 antibody for Flow Cytometry (Clone: NCI m971):

The Quest for Anti-α-Synuclein Antibody Specificity—Lessons Learnt From Flow Cytometry Analysis
Leupold L, et al. Front Neurol. 2022 Jul 20;13:869103. doi: 10.3389/fneur.2022.869103. PMID: 35911883
The NCI m971 antibody was evaluated for specificity in flow cytometry using a CytoFLEX flow cytometer, with data analyzed via FlowJo 8.5.3 or CytExpert software. Intracellular staining of 300,000 fixed cells involved permeabilization and blocking to minimize non-specific binding, with stain index and MFI ratios confirming optimal staining efficiency for α-synuclein detection.
Tags: activity of NCI m971 antibody; function of NCI m971 antibody

Evaluation of the Anti-Tumor Activity of the Humanized Monoclonal Antibody NEO-201 in Preclinical Models of Ovarian Cancer
Zeligs KP, et al. Front Oncol. 2020 May 12;10:805. doi: 10.3389/fonc.2020.00805. PMID: 32477371
The NCI m971 antibody (NEO-201), conjugated to Pacific Blue or PE, was used in flow cytometry to analyze tumor antigen expression on ASPC-1, BxPC3, CFPAC-1, and LS174T cell lines, showing overlap with CEACAM5 and CEACAM6. ELISA confirmed binding to recombinant CEACAM5 and CEACAM6 but not CEACAM1 or CEACAM8, with IHC verification except in PEO1 cells.
Tags: NCI m971 antibody; NCI m971 antibody for animal model

Structural details of monoclonal antibody m971 recognition of the membrane-proximal domain of CD22
Ereño-Orbea J, et al. J Biol Chem. 2021 Oct;296:100966. doi: 10.1016/j.jbc.2021.100966. PMID: 34273351
The NCI m971 antibody’s Fab fragment bound the CD22 d7 domain with low nanomolar affinity (25 nM by BLI, comparable to 75 nM by flow cytometry). Cocrystal structures showed a preconfigured paratope (RMSD 0.33 Å), with HCDRs 1–3 and LCDRs 1–2 interacting with CD22’s C β-strand and loops, unaffected by N-linked glycosylation.
Tags: NCI m971 antibody for flow cytometry; NCI m971 antibody

Validation of a Flow Cytometry Based Binding Assay for Evaluation of Monoclonal Antibody Recognizing EGF Receptor
Cedeño-Arias M, et al. Sci Pharm. 2011 Jul-Sep;79(3):603-15. doi: 10.3797/scipharm.1104-18. PMID: 21886904
The NCI m971 antibody was used in a flow cytometry binding assay on a Becton Dickinson FACSCan with 488 argon lasers to characterize receptor interactions. Cells were stained in PBS + 1% BSA, with the assay’s robustness validated across two cell lines, ensuring accurate quantification of binding activity.
Tags: NCI m971 mAb for animal model; NCI m971 mAb for flow cytometry

Flow cytometric screening of monoclonal antibodies for drug or toxin targeting to human cancer
Durrant LG, et al. J Natl Cancer Inst. 1989 May 3;81(9):688-95. doi: 10.1093/jnci/81.9.688. PMID: 2468779
The NCI m971 antibody (NCRC-23, anti-carcinoembryonic antigen) was evaluated by flow cytometry, recognizing 65% of large colorectal tumor cells with a mean antigen density of 4.9 × 10^5 sites/cell. Combinations with other antibodies improved targeting coverage, reducing tumor recurrence risk in primary tumor samples.
Tags: NCI m971 mAb; NCI m971 monoclonal antibody

For more references about anti-human Siglec-2/CD22 antibody (NCI m971), please contact our scientific support team with message@sydlabs.com.