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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: NCI m971.
Isotype: Mouse IgG2a kappa.
Source: The recombinant anti-human Siglec-2/CD22 monoclonal antibody (clone: NCI m971) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant mouse monoclonal antibody (clone: NCI m971) specifically binds to the human Siglec-2/CD22 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant human IgG1 monoclonal antibody with Fc silent mutation.
Clone: NCI m971.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The recombinant anti-human Siglec-2/CD22 monoclonal antibody (clone: NCI m971) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant NCI m971 antibody specifically binds to the human Siglec-2/CD22 protein.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: NCI m971.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-human Siglec-2/CD22 monoclonal antibody (clone: NCI m971) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant NCI m971 antibody specifically binds to the human Siglec-2/CD22 protein.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The NCI m971 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Syd Labs provides the following recombinant anti-human Siglec-2/CD22 monoclonal antibodies for flow cytometry:
Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
Biotin Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
FITC Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
APC Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PE Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
Pacific Blue Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PerCP Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PE/Cy5 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PE/Cy7 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 488 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 549 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 594 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 647 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 700 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
APC/Cy7 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PerCP/Cy5.5 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
Background:
The recombinant NCI m971 antibody binds to the human Siglec-2/CD22 protein, a sugar binding transmembrane protein, which specifically binds sialic acid with an immunoglobulin (Ig) domain located at its N-terminus. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice. In mice, it has been shown that CD22 blockade restores homeostatic microglial phagocytosis in aging brains.
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