PA007619.r2a:In Vivo Grade Recombinant Anti-human IL-10 Monoclonal Antibody (Clone: JES3-19F1.1.1), Rat IgG2a Kappa
Recombinant Rat IgG2a Monoclonal Antibody.
Clone: JES3-19F1.1.1.
Isotype: Rat IgG2a Kappa.
Source:The anti-human IL-10 monoclonal antibody (clone: JES3-19F1.1.1) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: JES3-19F1.1.1) specifically binds to human IL-10.
Applications:ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human IL-10 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-human IL-10 monoclonal antibody of clone JES3-19F1.1.1 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-human IL-10 antibody (Clone: JES3-19F1.1.1):
IL-10R inhibition reprograms tumor-associated macrophages and reverses drug resistance in multiple myeloma
Vannini C, et al. Nat Cancer. 2024 Sep 5. doi: 10.1038/s43018-024-00815-0. PMID: 39215060
The anti-IL-10R antibody (clone JES3-19F1.1.1) was used to block IL-10 signaling in the tumor microenvironment of a multiple myeloma mouse model, significantly inhibiting tumor growth in combination therapy. Flow cytometry showed increased M1-like macrophage polarization, and survival curves indicated prolonged median survival compared to controls.
Tags: anti-human IL-10 JES3-19F1.1.1; anti-human IL-10 JES3-19F1.1.1 antibody
IL-10 constrains sphingolipid metabolism to limit inflammation
Harris HE, et al. Nature. 2024 Feb 21;626(7999):604-611. doi: 10.1038/s41586-024-07078-0. PMID: 38383790
The JES3-19F1.1.1 anti-IL-10R antibody, administered intraperitoneally, elevated ceramide levels in colonic tissue of colitis-induced mice. Inflammatory scores decreased with sphingolipid modulation, and gene expression profiling revealed upregulated pathways in treated animals, indicating a role in limiting inflammation.
Tags: anti-human IL-10 JES3-19F1.1.1 in vivo; anti-human IL-10 JES3-19F1.1.1 in animal model
IL-10R blockade during chronic schistosomiasis mansoni results in the loss of B cells from the liver and the development of severe pulmonary disease
Wynn TA, et al. PLoS One. 2012 Jan 11;7(1):e30040. doi: 10.1371/journal.pone.0030040. PMID: 22291593
The JES3-19F1.1.1 antibody neutralized IL-10R during chronic Schistosoma mansoni infection in mice, leading to hepatic B cell depletion 4 weeks post-treatment. Pulmonary inflammation scores increased significantly, with histopathology showing granuloma formation in the lungs of treated mice.
Tags: anti-human IL-10 JES3-19F1.1.1 mAb in animal model; anti-human IL-10 JES3-19F1.1.1 in cancer research
IL-10 restricts activation-induced death of NK cells during acute murine cytomegalovirus infection
Shellam GR, et al. PLoS One. 2011 Aug 25;6(8):e24500. doi: 10.1371/journal.pone.0024500. PMID: 21849677
The JES3-19F1.1.1 anti-IL-10R antibody, injected in mice infected with murine cytomegalovirus, reduced viral titers in the spleen and decreased NK cell apoptosis rates. Cytokine profiles shifted towards pro-inflammatory responses, highlighting the antibody’s role in modulating NK cell survival.
Tags: bioactivity of anti-human IL-10 JES3-19F1.1.1; anti-human IL-10 JES3-19F1.1.1 of low endotoxin
IL-10 and TGF-beta redundantly protect against severe liver injury and mortality during acute schistosomiasis
Wynn TA, et al. J Immunol. 2009 Jan 15;182(2):1150-60. doi: 10.4049/jimmunol.181.2.1150. PMID: 18981143
Combined JES3-19F1.1.1 and anti-TGF-β antibodies in acute schistosomiasis mouse models reduced liver fibrosis and mortality from 90% to 40%. Liver histopathology showed smaller granulomas in treated cohorts, demonstrating the protective role of dual IL-10R and TGF-β blockade.
Tags: anti-IL-10 (JES3-19F1.1.1) monoclonal antibody; clone JES3-19F1.1.1 of human IL-10 antibody
For more references about anti-human IL-10 antibody (JES3-19F1.1.1), please contact our scientific support team with message@sydlabs.com.