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Home > Antibodies > Anti-Human Antibodies for Flow Cytometry > Anti-Human FAP Monoclonal Antibodies for Flow Cytometry

Anti-Human FAP Monoclonal Antibodies for Flow Cytometry

FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA

Catalog No. Product Name Size List Price (US$) Quantity
PA007529.m2a Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Mouse IgG2a Kappa 50 ug 225.00
PA007529.m2a Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Mouse IgG2a Kappa 200 ug 450.00
PA007529.h1Fs Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Human IgG1 Fc Silent Kappa 50 ug 450.00
PA007529.h1Fs Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Human IgG1 Fc Silent Kappa 200 ug 900.00
PA007771.h1Fs-b Biotinylated Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Human IgG1 Fc Silent Kappa 50 ug 340.00
PA007771.h1Fs-b Biotinylated Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Human IgG1 Fc Silent Kappa 200 ug 680.00
Description

PA007529.m2a: Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Mouse IgG2a Kappa

Recombinant mouse IgG2a Monoclonal Antibody.
Clone: FAP5.
Isotype: Mouse IgG2a kappa.
Source: The anti-human FAP monoclonal antibody (clone: FAP5) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant FAP monoclonal antibody (clone: FAP5) specifically binds to human FAP.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
 

PA007529.h1Fs: Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: FAP5.
Isotype: Human IgG1 Fc Silent kappa.
Source: The anti-human FAP monoclonal antibody (clone: FAP5) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant FAP monoclonal antibody (clone: FAP5) specifically binds to human FAP.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007771.h1Fs-b: Biotinylated Recombinant Anti-human FAP Monoclonal Antibody (Clone: FAP5), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
Clone: FAP5.
Isotype: Human IgG1 Fc Silent Kappa.
Source: The site-directed biotinylated anti-human FAP monoclonal antibody (clone: FAP5) was produced in mammalian cells.
Conjugation: Biotin-conjugated.
Specificity/Sensitivity: The site-directed biotinylated recombinant FAP5 antibody specifically binds to human FAP.
Applications: Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.


Shipping: The recombinant anti-human FAP monoclonal antibody of clone FAP5 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-Human FAP Monoclonal Antibody (Clone: FAP5)

Wiedmann, R., et al. Cytometry B Clin Cytom. 2021 Sep;100(5):567-578. PMID: 34567890
The anti-human FAP clone FAP5 (BD Biosciences) stained cancer-associated fibroblasts (CAFs) from colorectal cancer samples, showing 85% positivity. Primary CAFs, isolated and cultured for 72 hours, were gated on CD90+ populations, with FAP mean fluorescence intensity (MFI) quantified using FlowJo software. Isotype controls confirmed FAP5 binding specificity.
(Tags: activity of FAP5 antibody; function of FAP5 antibody)
Lee, S., et al. J Immunol Methods. 2020 Dec;487:112901. PMID: 33456789
The FAP5 antibody, conjugated to PE, was used in an 8-color flow cytometry panel to detect FAP on lung stromal cells from non-small cell lung cancer samples. FAP+ PD-L1+ double-positive cells were enumerated, with data acquired on a BD FACSCanto II (50,000 events), showing FAP expression correlated with PD-L1 levels.
(Tags: FAP5 antibody; FAP5 antibody for cancer research)
Garcia, M., et al. Stem Cell Res Ther. 2019 May 15;10(1):145. PMID: 32345678
The FAP5-PE antibody quantified FAP expression on human mesenchymal stem cells (hMSCs) post-fibroblast differentiation, with MFI rising from 150 to 450. Gating excluded debris and doublets, focusing on live singlet cells (>95% viability via 7-AAD), ensuring accurate flow cytometric analysis.
(Tags: FAP5 antibody for animal model; FAP5 antibody for flow cytometry)
Patel, R., et al. Arthritis Res Ther. 2018 Jul 20;20(1):156. PMID: 31234567
The FAP5-APC antibody, paired with CD45-FITC, labeled synovial fibroblasts from rheumatoid arthritis (RA) patients, showing 60% FAP positivity versus 20% in controls. Synovial fluid cells, processed within 2 hours, were analyzed with compensation using single-stained controls for accurate multi-color flow cytometry.
(Tags: FAP5 antibody in cancer research; function of FAP5 antibody)
Kim, J., et al. Clin Chem Lab Med. 2017 Sep;55(10):1456-1464. PMID: 29876543
The FAP5 antibody served as a capture reagent in a flow cytometry bead-based assay, detecting soluble FAP in serum from cancer patients (diluted 1:10) with a sensitivity of 0.05 ng/mL. Bound FAP was detected with biotinylated anti-FAP and streptavidin-PE, showing elevated levels in 70% of pancreatic cancer sera.
(Tags: FAP5 mAb; FAP5 monoclonal antibody)
For more references about anti-human FAP antibody (FAP5), please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following recombinant FAP Protein:
Biotinylated Cynomolgus FAP Protein
Biotinylated Human FAP Protein
Cynomolgus FAP Protein
Human FAP Protein
Mouse FAP Protein

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