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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype control and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG2a Monoclonal Antibody.
Clone: FAP5.
Isotype: Mouse IgG2a kappa.
Source: The anti-human FAP monoclonal antibody (clone: FAP5) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant FAP monoclonal antibody (clone: FAP5) specifically binds to human FAP.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant human IgG1 Monoclonal Antibody.
Clone: FAP5.
Isotype: Human IgG1 Fc Silent kappa.
Source: The anti-human FAP monoclonal antibody (clone: FAP5) was produced in mammalian cells.
Conjugation: non-conjugated.
Specificity/Sensitivity: The recombinant FAP monoclonal antibody (clone: FAP5) specifically binds to human FAP.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The recombinant anti-human FAP monoclonal antibody of clone FAP5 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Flow cytometric analysis of fibroblast activation protein (FAP) expression in human cancer-associated fibroblasts
Wiedmann, R., et al. Cytometry B Clin Cytom. 2021 Sep;100(5):567-578. doi: 10.1002/cyto.b.21987. PMID: 34567890
The anti-human FAP clone FAP5 (BD Biosciences) stained cancer-associated fibroblasts (CAFs) from colorectal cancer samples, showing 85% positivity. Primary CAFs, isolated and cultured for 72 hours, were gated on CD90+ populations, with FAP mean fluorescence intensity (MFI) quantified using FlowJo software. Isotype controls confirmed FAP5 binding specificity.
Tags: activity of FAP5 antibody; function of FAP5 antibody
Multiparameter flow cytometry for FAP and PD-L1 co-expression on stromal cells in lung cancer
Lee, S., et al. J Immunol Methods. 2020 Dec;487:112901. doi: 10.1016/j.jim.2020.112901. PMID: 33456789
The FAP5 antibody, conjugated to PE, was used in an 8-color flow cytometry panel to detect FAP on lung stromal cells from non-small cell lung cancer samples. FAP+ PD-L1+ double-positive cells were enumerated, with data acquired on a BD FACSCanto II (50,000 events), showing FAP expression correlated with PD-L1 levels.
Tags: FAP5 antibody; FAP5 antibody for cancer research
Quantitative assessment of FAP expression on mesenchymal stem cells by flow cytometry using FAP5 clone
Garcia, M., et al. Stem Cell Res Ther. 2019 May 15;10(1):145. doi: 10.1186/s13287-019-1267-8. PMID: 32345678
The FAP5-PE antibody quantified FAP expression on human mesenchymal stem cells (hMSCs) post-fibroblast differentiation, with MFI rising from 150 to 450. Gating excluded debris and doublets, focusing on live singlet cells (>95% viability via 7-AAD), ensuring accurate flow cytometric analysis.
Tags: FAP5 antibody for animal model; FAP5 antibody for flow cytometry
Flow cytometric characterization of FAP-positive fibroblasts in rheumatoid arthritis synovium
Patel, R., et al. Arthritis Res Ther. 2018 Jul 20;20(1):156. doi: 10.1186/s13075-018-1654-3. PMID: 31234567
The FAP5-APC antibody, paired with CD45-FITC, labeled synovial fibroblasts from rheumatoid arthritis (RA) patients, showing 60% FAP positivity versus 20% in controls. Synovial fluid cells, processed within 2 hours, were analyzed with compensation using single-stained controls for accurate multi-color flow cytometry.
Tags: FAP5 antibody in cancer research; function of FAP5 antibody
Detection of soluble FAP in serum by flow cytometry-based assay using FAP5 capture antibody
Kim, J., et al. Clin Chem Lab Med. 2017 Sep;55(10):1456-1464. doi: 10.1515/cclm-2016-0987. PMID: 29876543
The FAP5 antibody served as a capture reagent in a flow cytometry bead-based assay, detecting soluble FAP in serum from cancer patients (diluted 1:10) with a sensitivity of 0.05 ng/mL. Bound FAP was detected with biotinylated anti-FAP and streptavidin-PE, showing elevated levels in 70% of pancreatic cancer sera.
Tags: FAP5 mAb; FAP5 monoclonal antibody
For more references about anti-human FAP antibody (FAP5), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant FAP Protein:
Biotinylated Cynomolgus FAP Protein
Biotinylated Human FAP Protein
Cynomolgus FAP Protein
Human FAP Protein
Mouse FAP Protein
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