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Conditions of optimal Alkaline Phosphatase performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.
Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
BP000766-C554: Recombinant Human Alkaline Phosphatase (ALPL) Enzyme
Source: HEK 293?derived.
Recombinant Human Alkaline Phosphatase (ALPL) Enzyme is expressed with sequence (Leu18-Ser502) of Human ALPL fused with a polyhistidine tag at the C-terminus.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 EU/ug).
Formulation: 0.2 um filtered solution in 20 mM HEPES, 150 mM Nacl, 2 mM MgSO4, 0.1 mM ZnCl2, pH7.5.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
Other ALPL Enzymes:
Bovine Intestinal ALPL Enzyme
ALPL Antibodies:
ALPL Polyclonal Antibody
ALPL Polyclonal Antibody
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