Note: Price/availability/specifications subject to change without notice. If not specified, all products and services are for in vitro research use only. Not for use in or on humans or animals.
Order offline: Phone: 1-617-401-8149, Fax: 1-617-606-5019, Email: message@sydlabs.com, or leave a message with a formal purchase order (PO) or credit card.

Features:

PA000001-E0T502: His-Tag Polyclonal Antibody

Rabbit Polyclonal Antibody;
Applications: WB;
Reactivity: Human, Mouse, Rat, Others.

PA000001-E1T504: His-Tag Monoclonal Antibody

Mouse Monoclonal Antibody;
Applications: WB, Immunofluorescence, Immunoprecipitation;
Reactivity: Human, Mouse, Rat, Others;
Clone: HIS.H8;
Isotype: IgG2b.

PA000001-M20001: His-Tag (2A8) Monoclonal Antibody

Mouse Monoclonal Antibody;
Applications: ELISA, Immunofluorescence, Immunoprecipitation, WB;
Reactivity: Human. and Others;
Clone: HIS. 2A8;
Isotype: IgG1.

PA000001-P-205: His-Tag Monoclonal Antibody

Mouse Monoclonal Antibody;
Applications: Western Blot, Immunoprecipitation,Immunostaining;
Reactivity: All Species.
Clone: HIS.H8;
Isotype: IgG2b.

PA000001-P-225: HRP-conjugated His-Tag Polyclonal Antibody

Rabbit Polyclonal Antibody;
Applications: ELISA, Western Blot;
Reactivity: All Species;
Conjugation: Reductive amination, HRP/Ab Ratio = 5/1.

PA000001-P-236: His-Tag Polyclonal Antibody

Rabbit Polyclonal Antibody;
Applications: ELISA, Western Blot, Immunofluorescence, Immunoprecipitation;
Reactivity: All Species.

Description:

Background
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.

PA000001-E0T502: His-Tag Polyclonal Antibody

Form of Antibody: Rabbit IgG in PBS (without Mg2+ and Ca2+), pH 7.4, with 150 mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen: 6x His synthetic peptide.
Purification: Affinity purified with epitope-specific peptide on affinity-chromatography.
Specificity/Sensitivity: His-Tag polyclonal antibody recognizes His-tagged proteins overexpressed in cells, including both amino-or carboxy-termini of targeted proteins in transfected mammalian cells.
Applications: Western Blot 1:500-1:1000.

PA000001-E1T504: His-Tag Monoclonal Antibody

Form of Antibody: 1 mg/ml in PBS, pH 7.4, with 0.05% sodium azide.
Immunogen: 6x His synthetic peptide.
Specificity/Sensitivity: His-tag monoclonal antibody recognizes His-tagged tagged proteins overpressed in cells, including both amino-or carboxy-termini of targeted proteins in transfected mammalian cells.
Applications: Western Blot 1:1000-4000, Immunoprecipitation 1:200-400, immunostaining 1:200-400.

PA000001-M20001: His-Tag (2A8) Monoclonal Antibody

Form of Antibody: Mouse IgG in PBS, pH 7.4, with 0.05% sodium azide.
Immunogen: 6× His peptide conjugated to KLH.
Specifity: His-Tag (2A8) Mouse Monoclonal Antibody recognizes over-expressed or recombinant proteins containing the 6× His epitope tag.
Applications: Western blotting 1:5000, Immunofluorescence 1:2000, Immunoprecipitation 1:100, ELISA 1:2000.

PA000001-P-205: His-Tag Monoclonal Antibody

Form of Antibody: 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide.
Immunogen: 6x His synthetic peptide.
Specificity/Sensitivity: His-tag Monoclonal Antibody recognizes His-tagged recombinant proteins or His-tagged proteins over-expressed in cells.
Applications: Western Blot 1:1000, Immunoprecipitation 1:200, Immunostaining 1:200.

PA000001-P-225: HRP-conjugated His-Tag Polyclonal Antibody

Form of Antibody: 500 mg/ml in HRP stabilizing buffer.
Immunogen: 6× His peptide conjugated to KLH.
Purification: Affinity purified with HHHHHHH peptide on Agarose.
Applications: ELISA 0.5 μg/ml, Western Blot 0.5 μg/ml.

PA000001-P-236: His-Tag Polyclonal Antibody

Form of Antibody: 1 mg/ml in TBS with 0.01% NaN3.
Immunogen: 6x His peptide conjugated to KLH.
Purification: Affinity purified with HHHHHHH peptide on Agarose.
Applications: ELISA 0.5 μg/ml, Western Blot 1 μg/ml, Immunoprecipitation 5 μg/mg crude protein, Immunofluorescence 2 μg/ml.

Storage/Stability: Stable for 1 year at -20°C and 3 months at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Related Links:

• Human Recombinant Adiponectin with His Tag
• Human Recombinant APPL1 with His-tag
• Recombinant Human APPL2 with His-tag
• Guaranteed Mouse Monoclonal Antibody Development Services
• Compare our products with those from other vendors on Ushelf.com