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Features:

BP000030-GD4: Recombinant Human FGF Basic 147

E. coli ­derived.
The FGF basic (bFGF/FGF-2/FGF2) activity is measured in a cell proliferation assay using NIH­-3T3 mouse fibroblasts. The ED50 for this effect is less than 0.5 ng/ml.
Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method.
Purity > 97%, by SDS­-PAGE under reducing conditions and visualized by silver stain.

Description:

Background

FGF basic (bFGF/FGF-2/FGF2) is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic is produced by epithelial, tumor and other cell types (1). FGF basic protein (bFGF/FGF-2/FGF2) is involved in developmental processes and regulates differentiation, proliferation, and migration. FGF basic protein (bFGF/FGF-2/FGF2) is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic protein (bFGF/FGF-2/FGF2) has a high affinity for heparan sulfate (2) and binding is a step in the FGF basic activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic protein (bFGF/FGF-2/FGF2) binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways.

References:

1. Thomas, K.A. and Gimenz-Gallego, G. (1986) Trends Biochem. Sci. 11, 81.Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
2. Rifkin, D. and Moscatelli, D. (1989) J. Cell Biol. 109, 1.

BP000030-GD4: Recombinant Human FGF Basic 147

Source: E. coli ­derived, Leu142-Ser288, with an N-terminal Gly, Accession# NP_001997
N-terminal sequence analysis: Gly-Leu142
Predicted molecular mass: 16.0 kDa
SDS­-PAGE: 17 kDa, reducing conditions
The FGF basic protein (bFGF/FGF-2/FGF2) activity is measured in a cell proliferation assay using NIH­-3T3 mouse fibroblasts. The ED50 for this effect is less than 0.5 ng/ml.
Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method.
Purity > 97%, by SDS­-PAGE under reducing conditions and visualized by silver stain.
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.2.
Reconstitution: Reconstitute at 10 ug/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Shipping: The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-­thaw cycles.
12 months from date of receipt, -20 to -­70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

Related Links:

• aFGF Protein
• FGF Acidic/aFGF/FGF-1/FGF1 Antibody
• FGF Basic/bFGF/FGF-2/FGF2 Antibody
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• FGF products