Note: Price/availability/specifications subject to change without notice. If not specified, all products and services are for in vitro research use only. Not for use in or on humans or animals.
Order offline: Phone: 1-617-401-8149, Fax: 1-617-606-5019, Email: message@sydlabs.com, or leave a message with a formal purchase order (PO) or credit card.

Features:

Ease of Use:

* Faster sample preparation
* Minimize loss of protein
* Optional adoption, no re-validation required
* Ready-to-go "out of the box", no plate coating required
* Plug and play for process development, process monitoring, and quality control
* No individual reagent qualification
* No methods development required

EK000106-9000-1: Protein A ELISA kit for detection of natural and recombinant Protein A

* Sensitivity of 0.1 ng/mL (0.8ppm)
* Accuracy/Recovery ± 80-120%
* Precision: Inter-assay 10%, Intra-assay 15%
* Specificity for all variants of natural and recombinant Protein A
* 2 hour assay completion time

EK000106-9333-1: Protein A ELISA kit for detection of MabSelect SuReTM ligand

* Sensitivity of 0.1 ng/mL
* Accuracy/Recovery ± 80-120%
* Precision: Inter-assay 10%, Intra-assay 15%
* Specificity for MabSelect SuReTM ligand
* 2 hour assay completion time

Description:

Protein A ELISA Kits for detection of leached Protein A are chosen by biopharmaceutical companies for their easy-to-use procedure, quick run time, sensitivity and accurate results. Our kits are used in process development, process monitoring, and in final product testing to quantify natural, recombinant Protein A, and the MabSelect SuReTM* ligand contamination in samples eluted off of a Protein A chromatography column.

The combination of a specific capture system and an optimized sample diluent yields a highly sensitive assay able to accurately detect Protein A or SuReTM in the presence of antibodies without the time and resources required for in-house assay development and optimization.

Immunological assays are the "gold standard" for quantifying Protein A, and it is well recognized in the quality organizations that for the most accurate results the best practice is to incorporate an internal standard [and spiking protein] that is most like the contaminant ligand. For this reason we offer two distinct Protein A detection assays, each kit with its own unique standard:

* one for the detection of natural and recombinant Protein A
* another for the detection of the GE Healthcare MabSelect SureTM ligand.

NEW Dilute-&-Go Protocol: Speeds up and further simplifies our Protein A ELISA assay procedure by eliminating dialysis or buffer exchange for most sample matrices. This reduces sample preparation time, minimizes protein loss and makes the kits very easy to use!

EK000106-9000-1: Protein A ELISA kit for detection of natural and recombinant Protein A

The Protein A ELISA kits have been used by technology leaders in our industry for over 10 years to ensure lot-to-lot consistency in monoclonal antibody and Ig fusion products. This kit is appropriate for processes that run columns that contain any Protein A resin.

EK000106-9333-1: Protein A ELISA kit for detection of MabSelect SuReTM ligand

For downstream processes that utilize GE Healthcare’s base stable affinity media MabSelect SuReTM, it includes the SuReTM ligand standard for the most accurate spiking and recovery results.

Related Links:

• Protein A
• Protein A Beads
• Protein A Pre-Packed Columns
• Free ELISA Kit Samples
• ELISA Kits for Human, Mouce, and Rat Proteins