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Hybridoma antibody cloning and sequencing protocols

The general hybridoma antibody cloning protocol and antibody sequencing protocol seem very straightforward: RNA extraction, reverse transcription to cDNA, nested PCR amplifications, cloning of PCR products, sequencing, and analysis of sequences. However, it has taken scientists a long time to optimize a variety of parameters in the protocols, especially V-gene specific primers and antibody sequence […]

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Single B cell antibody cloning and sequencing protocols

Similar to process of hybridoma or clonal B cell antibody cloning and sequencing, the work flow of single B cell antibody cloning and sequencing is: separation of peripheral blood mononuclear cells (PBMC) from blood, staining of PBMC with the B cell selective marker (CD19 antibody), sorting of stained B cells by flow cytometry, total RNA […]

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High-throughput antibody repertoire sequencing protocols

Two mainstream strategies have been used to sequence antibody repertoires. The classical strategy is to design primers to cover as many functional V-genes as possible and create a diverse antibody libraries. The modern strategy is to use high-throughput next generation sequencing (NGS) to “deeply” sequencing of binding populations. The abundance of antibody mRNA can also […]

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FAQs about Antibody Sequencing Services

Antibody sequencing service (antibody cloning service, hybridoma cloning service, hybridoma sequencing service): – How to prepare samples for antibody sequencing services? – What is included in the study report of antibody sequencing projects?

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How to prepare samples for antibody sequencing services?

Three ways to submit samples for antibody sequencing: – Freeze the antibody producing cells, and send us a frozen vial on dry ice. If you have frozen stocks, you can directly ship the stock to us or extract RNAs from the stocks. It is unnecessary to grow new cells. –  Purify total RNAs from the […]

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What is included in the study report of antibody sequencing projects?

Our standard antibody sequencing procedure gives us the full variable region and part of the constant region of heavy and light chains. We provide an antibody sequence analysis report specifying the number of heavy and light chains identified, analysis of the results, agarose gel images of the amplified DNA fragments, and the DNA and protein […]

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Antibody Cloning and Sequencing Service for $1200 only

Syd Labs clones and sequences both heavy chain variable region (VH) and light chain variable region (VL) of monoclonal antibodies from $1200 per hybridoma, a market leading price. More features of our services of antibody cloning and sequencing: 1). Fast turnaround time: standard 2-3 weeks, with an industry record, 4 days; 2). 100000 cells per […]

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Hamster Hybridoma Antibody Sequencing Service

Hamster hybridoma antibody cloning and antibody sequencing is the most challenging among hybridoma antibody cloning and sequencing because sparse sequence and structural information for hamster antibodies is available in public sources. Back to 2011 when Syd Labs started to provide the services of hamaster hybridoma antibody cloning and antibody sequencing, one could find in Genbank […]

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