The cheapest but easy and effective way to get a plasmid miniprep kit is to buy spin columns for plasmid miniprep alone and make solutions with the following buffer recipe: Buffer P1: 50 mM Tris-HCl, 10 mM EDTA, pH 8.0 (25°C, the same below), 50 ug/ml RNase A Buffer P2: 0.2 M NaOH, 1% SDS […]
Plasmid miniprep protocols (spin columns)
Most of commercial plasmid miniprep kits rely on the spin column with silica membrane. The plasmid DNAs purified can be used for routine molecular biology applications such as DNA subcloning, PCR, in vitro transcription, DNA sequencing, and DNA transfection into mammalian cells. Protocols of plasmid miniprep using spin columns: – QIAprep Spin Miniprep Kit High-Yield […]
Free Samples of Spin Column for Plasmid Miniprep, PCR Product Cleanup, and Agarose Gel Extraction
Syd Labs universal spin columns (catalog No. MB000082 or MB082) can be used for DNA extraction and purification, such as plasmid miniprep, PCR cleanup, and agarose gel extraction. The yield using our universal spin columns is the same as or similar to those from top manufacturers. Welcome to contact Syd Labs and ask for our […]