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Buffer recipe for plasmid miniprep using spin columns

The cheapest but easy and effective way to get a plasmid miniprep kit is to buy spin columns for plasmid miniprep alone and make solutions with the following buffer recipe: Buffer P1: 50 mM Tris-HCl, 10 mM EDTA, pH 8.0 (25°C, the same below), 50 ug/ml RNase A Buffer P2: 0.2 M NaOH, 1% SDS […]


Plasmid miniprep protocols (spin columns)

Most of commercial plasmid miniprep kits rely on the spin column with silica membrane. The plasmid DNAs purified can be used for routine molecular biology applications such as DNA subcloning, PCR, in vitro transcription, DNA sequencing, and DNA transfection into mammalian cells. Protocols of plasmid miniprep using spin columns: – QIAprep Spin Miniprep Kit High-Yield […]