The general hybridoma antibody cloning protocol and antibody sequencing protocol seem very straightforward: RNA extraction, reverse transcription to cDNA, nested PCR amplifications, cloning of PCR products, sequencing, and analysis of sequences. However, it has taken scientists a long time to optimize a variety of parameters in the protocols, especially V-gene specific primers and antibody sequence […]
Single B cell antibody cloning and sequencing protocols
Similar to process of hybridoma or clonal B cell antibody cloning and sequencing, the work flow of single B cell antibody cloning and sequencing is: separation of peripheral blood mononuclear cells (PBMC) from blood, staining of PBMC with the B cell selective marker (CD19 antibody), sorting of stained B cells by flow cytometry, total RNA […]
High-throughput antibody repertoire sequencing protocols
Two mainstream strategies have been used to sequence antibody repertoires. The classical strategy is to design primers to cover as many functional V-genes as possible and create a diverse antibody libraries. The modern strategy is to use high-throughput next generation sequencing (NGS) to “deeply” sequencing of binding populations. The abundance of antibody mRNA can also […]
scFv phage library construction and screening protocols
The key for successful scFv phage library construction is efficient two-step PCR amplification of heavy chain (VH) and ligh chain (VL) variable regions. scFv phage library construction and scFv phage library screening protocols from public literatures: – Efficient amplification of light and heavy chain variable regions and construction of a non-immune phage scFv library. scFv […]
Antibody Humanization Protocols
Antibody Humanization Protocols: The technique of antibody humanization is attractive but challenging for most scientists in academia and industry. Experienced scientists at Syd Labs provide antibody humanization service if the project is difficult for you. Disclaimer: We index the protocols for your information only. It does not necessarily mean that we agree with […]
Mouse Monoclonal Antibody Development Protocols
Mouse monoclonal antibody development protocols: The procedure of mouse monoclonal antibody development sounds very straightforward. However, many factors affects the efficiency and success rate. Experienced scientists at Syd Labs provides cost competitive and reliable ELISA guaranteed mouse monoclonal antibody development services if you have no resource to make it. Disclaimer: We index the protocols […]
Rabbit Polyclonal Antibody Development Protocols
Rabbit Polyclonal Antibody Development Protocols: The procedure of rabbit polyclonal antibody development sounds very straightforward. However, many factors affects the efficiency and success rate. Experienced scientists at Syd Labs provides cost competitive and reliable ELISA guaranteed rabbit polyclonal antibody development services if you have no resource to make it. Disclaimer: We index the protocols […]
RNA Extraction Protocols (Spin Columns)
Protocols of commercially available and customized RNA extraction kits using spin columns: – SV Total RNA Isolation System Protocol (Promega) Disclaimer: We index the protocols for your information only. It does not necessarily mean that we agree with all of them. You rather than Syd Labs takes full responsibility for using any information described […]
Real Time PCR (qPCR) Protocols
Real-time PCR (qPCR) protocols: The procedure of real-time PCR (qPCR) sounds very straightforward. However, many factors affects the efficiency and success rate. Experienced scientists at Syd Labs provides cost competitive and reliable real-time PCR (qPCR) services if you have no resource to make it. Disclaimer: We index the protocols for your information only. It […]