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Buffer recipe for plasmid miniprep using spin columns

The cheapest but easy and effective way to get a plasmid miniprep kit is to buy spin columns for plasmid miniprep alone and make solutions with the following buffer recipe: Buffer P1: 50 mM Tris-HCl, 10 mM EDTA, pH 8.0 (25°C, the same below), 50 ug/ml RNase A Buffer P2: 0.2 M NaOH, 1% SDS […]