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PCR product cloning protocols

Three mainstream strategies have been used to clone and subclone PCR-amplified DNA fragments. The traditional method is to digest the PCR products with restriction enzymes and ligate the digested DNA fragments with a digested vector. The second ligation strategy is called “TA cloning” based on the feature of ends of PCR products.  The modern cloning strategy is to do ligation-independent ligation or homologous recombination. Protocols of cloning and subcloning DNA fragments amplified by PCR from public literatures:

Quick and clean cloning: a ligation-independent cloning strategy for selective cloning of specific PCR products from non-specific mixes.
The cloning vector shares homology with only one of the two PCR primers and with a sequence present in the insert, which is nested and non-overlapping with the other gene-specific PCR primer.

 

The procedure of PCR product cloning sounds very straightforward. However, many factors affects the efficiency and success rate. Experienced scientists at Syd Labs provide rapid DNA (including PCR products) cloning and subcloning service.

 

Disclaimer:
We index the protocols for your information only. It does not necessarily mean that we agree with all of them. You rather than Syd Labs takes full responsibility for using any information described here.