RNA extraction protocols of commercially available and customized TRIzol reagents from public literatures:
Life Technologies provides excellent instructions about RNA and RNA isolation.
– Discussion about the best protocol for RNA extraction from whole blood.
Comments from scientists who have used various commercial kits including TRIzol and RNeasy to extract RNA from whole blood.
– Efficient recovery of whole blood RNA – a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species.
Five commonly used RNA extraction methods (RiboPure, PAXgene, TRIzol LS, LeukoLOCK, and RNeasy) using whole blood sampled under varying conditions from 20 wild carnivores were compared.
– TRIZOL RNA extraction protocol from tissues and cell culture.
A TRIZOL-based protocol for RNA extraction from tissues and culture of cells.
– RNA extraction and purification protocols using both TRIZOL and RNeasy Mini kit.
Detailed protocols of RNA extraction and purification, using both TRIZOL reagent and spin column-based RNeasy Mini kit, and RNA quality control measurement.
– Cell total RNA preparation protocol.
Acid phenol-chloroform-isoamylalcohol (125:24:1, pH 4.3-4.7; Sigma P-1944) was used.
– RNA extraction protocol (guanidine thiocyanate/phenol method).
A modified protocol of single-step RNA extraction using guanidine thiocyanate/phenol.
– Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
The very basic method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture.
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