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MB095-V: pSL-T Simple Vector
For subcloning PCR products without enzymatic digestion.
The single 3' thymidine overhangs on both ends of the vector and at the insertion site greatly improve the efficiency of ligation of PCR products into the plasmid by preventing recircularization of the vectors and providing compatible overhangs for PCR products generated by certain thermostable polymerases such as Taq and Tth which often add a single deoxyadenosine to the 3'-end of the amplified fragments.
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