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Home > Molecular Biology > Cloning and Expression > First-Strand cDNA Synthesis > MMLV RT Enzyme

MMLV RT Enzyme

Moloney Murine Leukemia Virus Reverse Trancscriptase
Catalog No. Product Name Size List Price (US$) Quantity
MB036-MM MMLV Reverse Transcriptase 10,000 units 60.00
MB036-ENZ-310 MMLV Reverse Transcriptase 50,000 units 600.00
MB036-AM AMV Reverse Transcriptase 500 units 96.00
Description

MB036-AM: AMV Reverse Transcriptase

For DNA polymerization using the RNA molecule as the template, filling in and labeling the 3'-termini of DNA with 5'-protruding ends, and dideoxy DNA sequencing and RNA sequencing.

AMV Reverse Transcriptase (AMV RTase) catalyzes DNA polymerization using the RNA molecule as the template. It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+. AMV Reverse Transcriptase is used chiefly to transcribe mRNA into cDNA, which can then be cloned into vectors or amplified by PCR. It may also be used for filling in and labeling the 3'-termini of DNA with 5'-protruding ends in addition to dideoxy DNA sequencing and RNA sequencing. The enzyme possesses an intrinsic RNase H activity.

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