PA007702.m1: Recombinant Anti-Fluorescein Monoclonal Antibody (Clone: 4-4-20 (enhanced)), Mouse IgG1 Kappa
Recombinant mouse IgG1 Monoclonal Antibody.
Specificity/Sensitivity: The recombinant anti-Fluorescein monoclonal antibody (clone: 4-4-20 (enhanced)) specifically binds to Fluorescein.
Applications: Western Blot, Flow Cytometry.PA007703.h1: Recombinant Anti-FITC Monoclonal Antibody (Clone: 4M5.3), Human IgG1 Kappa
Recombinant Human IgG1 Monoclonal Antibody.
Specificity/Sensitivity: The recombinant anti-FITC monoclonal antibody (clone: 4M5.3) specifically binds to FITC.
Applications: ELISA, Western Blot, Flow Cytometry.20000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
Both Sanger sequencing-based and nanopore sequencing-based methods are available for antibody sequencing from hybridoma and clonal B cells. Sanger sequencing is labor intensive and time consuming so that it is difficult to reduce the cost for a large number of samples. However, nanopore sequencing can be used to reduce the cost signicantly, especially for a large number of samples.
Single B cell antibody sequencing has become popular for antibody discovery, especially for those difficult to obtain. After antigen specific single B cell sorting, the single B cells can be directly used for antibody seqencing or cultured for 2 weeks before antibody sequencing. Single B cell sequencing by next generation sequencing (NGS) including nanopore sequencing has been successfully applied to single B cell antibody technologies.
MB082-PC: Spin Column for Plasmid Miniprep
* Spin columns are compatible to reagents in plasmid miniprep kits from most of other manufacturers, such as Qiagen and Sigma. The yield is similar. Thus, clients do not have to waste reagents from plasmid miniprep kits.
* Low cost spin columns: $85 for 200 universal spin columns. A promotion is running: 20% discount of spin columns for plasmid miniprep as credit to purchase any other products or services on sydlabs.com from August 1 to October 31, 2022. Limited to new customers.
* Syd Labs provides the recipe of reagents for plasmid miniprep if you buy spin columns from us. Very simple to make the reagents. The shipping fee is much lower if you buy spin columns only rather than the whole DNA purification kits with heavy and bulky reagents. Save more to make your own buffer.MB075-PC: Spin Column for Plasmid Maxiprep
* No poisonous phenol etc.
* For clients' convenience, Syd Labs sell spin columns alone which are compatible to reagents in plasmid maxiprep kits from most of other manufacturers, such as Qiagen and Sigma. The yield is similar. Thus, clients do not have to waste reagents from plasmid maxiprep kits.
* Low cost spin columns: $70 for 10 spin columns. A promotion is running: 20% discount of spin columns for plasmid maxiprep as credit to purchase any other products or services on sydlabs.com from Jan. 2 to June 30, 2014. Limited to new customers.Orchestrating different elements essential for optimal mammalian gene expression.
Fast return: 2-3 weeks.
Having made thousands of mammalian expression constructs and tested them in cell culture and in vivo, we are highly experienced in designing expression constructs for superb expression.BP003056: Recombinant SARS-CoV-2 Spike S1-Fc-Avi Protein (S Protein S1 Subunit)
Source: mammalian cells.
Activity: Immobilized recombinant human ACE2, His Tag at 0.5 ug/ml (100 ul/well). Dose response curve for the recombinant SARS-CoV-2 Spike S1-Fc-Avi protein with the EC50 of 0.1 ug/ml determined by ELISA.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.BP003050: Recombinant SARS-CoV-2 Spike RBD-Fc-Avi (S Protein)
Source: mammalian cells.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.BP003053: Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi (NP Protein, N Protein)
Source: E.coli.
Purity: > 95% by PAGE under reduced condition.