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Conditions of optimal Biotin-AGE-BSA performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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Advanced glycation end products (AGEs) accumulate with age and at an accelerated rate in diabetes. AGEs bind cell-surface receptors including the receptor for AGE (RAGE). The fluorescence of Biotin-AGE-BSA was determined by fluorescence spectrometry with Ex./Em.=360/465 nm. The Biotin-AGE-BSA shows a 100-fold increase in fluorescence when compared to the control BSA. Browning of Biotin-AGE-BSA was determined by measuring the absorbance at 320 nm. The Bioin-AGE-BSA shows a 40 fold increase in absorbance when compared to the control BSA.
BP000042-MPR10: Biotin-AGE-BSA
Source: The AGE modified BSA was produced by reacting BSA with glucose under sterile conditions for 2 months followed by dialysis and purification according to the method described in Valentia et al. (2004). The AGE-BSA was biotinylated and then subjected to dialysis.
Formulation: Phosphate-buffered saline (PBS), 1 ug/ml.
Endotoxin: < 0.1 EU/ug AGE-BSA as determined by the LAL method.
Preservative: None.
QC tests: SDS-PAGE, MALDI-TOF, Fluorescence spectrometry, Absorbance at 320 nm and RAGE binding ELISA.
Activity: KD 50-20 nM, as measured by its binding to the immobilized RAGE in an ELISA binding assay.
Extent of AGE modification: 25-30 adducts per Biotin-AGE-BSA, as judged by free amine content analysis and MALDI-TOF analysis.
Extent of biotinylation: 6-7 biotins per AGE-BSA, as judged by HABA assay and MALDI-TOF analysis.
Sterility: 0.2 um membrane-filtered and packaged aseptically.
Storage and stability:
Upon receiving, store the product at 2-8°C for up to 1 month. For extended storage, aliquot the solution and freeze at -70°C or -20°C. Avoid repeated freezing and thawing.
Reference: Valencia et al. (2004) Anal. Biochem. 324:68-78
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