Human CD24 Protein

BP005038: Human CD24 Protein with the N-GST Tag

Source: The recombinant Human CD24 Protein, (amino acids Ser27-Gly59) fused with a GST tag at the N-terminus, was produced in E.coli cells.
Molecular Weight: The recombinant Human CD24 protein with the GST tag at the N-terminus has a predicted MW of 31.6 kDa same as Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD24 protein with the N-GST tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-CD24 Antibody protein with the N-hFc tag with the EC50 of 4.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD24 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP003511: Human CD24 Protein with the C-His-Avi Tag

Source: The recombinant Human CD24 Protein, (amino acids Ser27-Gly59) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CD24 protein with the His-Avi tag at the C-terminus has a predicted MW of 6.5 kDa. Due to glycosylation, the protein migrates to 23-33 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD24 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-CD24 Antibody protein with the C-hFc tag with the EC50 of 67.4 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD24 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005414: Human CD24 Protein with the C-hFc Tag

Source: The recombinant Human CD24 Protein, (amino acids Ser27-Gly59) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CD24 protein with the hFc tag at the C-terminus has a predicted MW of 29.9 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD24 protein with the C-hFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-CD24 Antibody protein with the C-hFc tag with the EC50 of 0.58 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD24 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005415: Human CD24 Protein with the C-mFc Tag

Source: The recombinant Human CD24 Protein, (amino acids Ser27-Gly59) fused with a mFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CD24 protein with the mFc tag at the C-terminus has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD24 protein with the C-mFc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-CD24 Antibody protein with the C-hFc tag with the EC50 of 0.28 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD24 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005471: Human CD24 Protein with the C-Llama Fc Tag

Source: The recombinant Human CD24 Protein, (amino acids Ser27-Gly59) fused with a Llama Fc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human CD24 protein with the Llama Fc tag at the C-terminus has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human CD24 protein with the C-Llama Fc tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Biotinylated Anti-CD24 Antibody protein with the C-hFc tag with the EC50 of 0.26 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human CD24 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

Shipping: The recombinant Human CD24 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.