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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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AAV8 titration ELISA kit for the quantitative determination of full and empty AAV8 capsids. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
EK0402: AAV8 Titration ELISA Kit
Application: for the quantitative determination of full and empty AAV8 capsids, including intact AAV8 Wild Type virions, AAV8 recombinant virions, assembled AAV8 virions, intact empty AAV8 capsids.
This assay is specific for AAV8 capsids.
Detection range: 1.0^7 capsids/mL - 6.5^8 capsids/mL
Sensitivity: 1.0^7 capsids/mL
Precision: CV < 10%
Kit components for the 96-well plate ELISA kit for full and empty AAV8 capsids (1x 96-well microplate):
1) Pre-coated Microplate: 96 well polystyrene microplates coated with monoclonal antibody specific for AAV8 capsids; 1x 96-well microplate
2) Detection Antibody: Biotinylated anti-AAV8 antibody which binds to AAV8 in standard and test samples; 1 vial (30 ul)
3) HRP Conjugate: Streptavidin Peroxidase conjugate that binds to detection antibody and catalyzes color development; 1 bottle (15 ml)
4) AAV8 Capsid Standard: Lyophilized AAV8 powder ; 2 vials (6.5E+08 capsids/vial)
5) 10X Assay Buffer: for diluting standards or samples. Dilute to 1X before use; 1 bottle (10 ml)
6) 20X Wash Buffer: containing PBS and Tween-20. Dilute to 1X before use; 2 bottles (15 ml each)
7) Color Reagent: tetramethylbenzidine (TMB); 1 bottle (15 ml)
8) Stop Solution: 0.5 N hydrochloric acid solution; 1 bottle (10 ml)
9) Product Insert: product description, assay protocol, material data sheet; 1 book.
Background
Empty AAV8 capsids lacking the transgene of interest cause unwanted immune response without providing the therapeutic benefit of the transgene. Alongside DNA and infectious titer, determination of the capsid titer is crucial for validating the efficacy and safety of your AAV gene therapy. Our titration kit detects full and empty AAV8 capsids.
The AAV8 titration ELISA kit uses sandwich ELISA to determine the titer of AAV8 capsids in the test sample. The capture AAV8 monoclonal antibody is pre-coated and treated on a 96-well reaction plate. AAV8 standard or sample is added to the pre-coated plate and specifically binds to the capture antibody. The biotinylated detection AAV8 antibody is then added to the plate to bind the immune complex. Next, the streptavidin HRP conjugate is added to react with the biotin molecules. The addition of TMB results in color changes and the amplitude of the color change is proportional to the amount of AAV8 capsids that specifically bind to the plate. The reaction is stopped with the addition of stop solution and the absorbance is measured at 450nm. The sample AAV8 capsid titer is calculated from the AAV8 standards titration curve.
AAV Titration ELISA Kits:
AAV2 Titration ELISA Kit
AAV5 Titration ELISA Kit
AAV8 Titration ELISA Kit
AAV9 Titration ELISA Kit
Nuclease:
Recombinant Dr. Nuclease
RNase A Enzyme
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