100000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.

- Reliable results. With optimized workflow and PCR primers, we have successfully cloned and sequenced >3000 antibodies from various species in the past 10 years. Syd Labs has become a reliable CRO supplier of antibody sequencing to biotech and biopharma companies, government agencies including FDA and NIH, and universities. Clients' samples of hybridoma cell lines or clonal B cells just need to meet 2 requirements: functional antibodies are secreted by the cells; enough RNA remains.
- Save time. One may learn how to clone and sequence antibody genes by studying hybridoma antibody cloning and antibody sequencing protocols that we collected from public literature. Generally speaking, it is more difficult to clone antibody genes from rodents than other species; among isotypes, it is the easiest to clone IgG genes, especially IgG1. A couple of companies sell such kits and PCR primers. However, it takes time to become a highly efficient and experienced professional for so many lab skills and bioinformatics analysis involved in such projects. In addition, there are some samples invaluable but difficult to work. Some service suppliers spend months but could not solve the problem, just wasting clients' samples and time.
- Save money. Since we process samples from multiple clients in the same time, our cost is lower than clients'. Our low cost molecular biology reagents further save clients' cost.

Fast turnaround: 8-10 weeks in total. Our antibody humanization process involves significant computational analysis, and thus is quite efficient and requires much less trial time.

A human antibody database and a proprietary method are used for analysis and design of humanized antibodies.

Nine humanized antibodies (each combination of 3 designed heavy chains and 3 designed light chains) are constructed and produced for antigen binding confirmation and affinity measurement.

- Any gene;
- Any host cell, including primary cell;
- Significantly improved efficiency of changing protein expression level compared to non-viral DNA transfection methods.

Fast turnaround:

Clonal stable cell line generation: 12-14 weeks.
Cell pool generation: 8-10 weeks.

Syd Labs has successfully developed human and monkey monoclonal antibodies using antigen specific single B cell-based antibody generation technologies. After detection and isolation of antigen specific memory B cells by flow cytometry-based single B cell sorting, the antibody variable regions (VH and VL) from single or clonal B cells are cloned and sequenced. Recombinant rabbit monoclonal antibodies are produced, purified, and tested for various applications, such as drug development and diagnostics.

Fast turnaround: 8-10 weeks once the polyclonal sera, PBMC, and antigen is available.

Experienced scientists working on antigen and antibody production, antibody engineering, lentivirus, and cell line development services for decades. >90% success rate of stable cell line generation.

Fast turnaround:

Antigen (peptide or protein) production: 3-6 weeks.
Mouse monoclonal antibody production: 20-24 weeks.
Screening phage or yeast libraries for fully human antibodies: 8-12 weeks.
Sequencing hybrioma antibody: 1-3 weeks.
Chimeric antibody production: 3-4 weeks.
Antibody humanization: 8-10 weeks.
Construction of CAR cassette, lentivirus packaging and production: 4-5 weeks.
T-cell activation, transduction, and CAR T-cell expansion: 4-5 weeks.
Confirmation of CAR expression, IFN-gamma ELISA, and cytotoxicity assay of transduced and expanded T-cells: 2 weeks.

Our retrovirus production services include: 1) construction of a retrovirus plasmid vector for expression or silencing of a gene; 2) retrovirus packaging; 3) retrovirus purification; 4) retrovirus titration.

We have always optimized our retrovirus production systems, including retrovirus plasmid construction and low endotoxin plasmid preparation, host cells for retrovirus packaging, cell culture medium and retrovirus packaging process, small and large scale retrovirus purification process.

Fast turnaround: 2-3 weeks.

Our complete custom lentivirus production services include: 1) lentivirus vector construction for expression or silencing of a gene; 2) lentivirus packaging; 3) lentivirus purification; 4) lentivirus titering.

We have always optimized our lentivirus production systems, including lentivirus plasmid construction and low endotoxin plasmid preparation, host cells for lentivirus packaging, cell culture medium and lentivirus packaging process, small and large scale lentivirus purification process.

Fast turnaround: 2-3 weeks.

We provide large selection of pre-made adenovirus and complete custom adenovirus production services from small crude scale to large purified scale, including: 1) construction of an adenovirus vector for expression or silencing of a gene; 2) generation of adenovirus; 3) amplification and purification of adenovirus.

Our adenovirus production team has constructed, packaged, amplified, and purified thousands of adenoviruses for worldwide clients in more than a decade. An optimized technology is used to simplify and accelerate adenoviral vector construction for researchers studying gene expression or gene silencing in mammalian systems.

Fast turnaround: 2-3 weeks.

Syd Labs determines the titer of lentiviruses and retroviruses (provided by customer or produced by Syd Labs) using HT1080 cells.

Fast return: 2 weeks.

Syd Labs determines the titer of lentiviruses and retroviruses (provided by customer or produced by Syd Labs) using HT1080 cells.

Fast return: 2 weeks.