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The kit is provided in a convenient Master Mix format. The 2 x One-step Mix contains optimized buffer system, dNTP and loading dye; One-step Enzyme Mix contains optimized ratio of 4G Reverse Transcriptase, Murine RNase inhibitor, and hot start Taq DNA polymerase.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The 4G One-step RT-PCR Kit is optimized to do reverse transcription (RT) and PCR in a single tube. Except RNA samples and primers, the 4G One-step RT-PCR kit contains all components of RT and PCR mixed in one tube before starting the reaction and thus, RT and PCR are carried out sequentially in the same tube. In the 4G One-step RT-PCR kit, the reverse transcriptase, hot-start Taq DNA polymerase, and 2x RT-PCR buffer are all optimized for both the RT and PCR steps. The 4G One-step RT-PCR kit is specially designed for end-point PCR detection using RNA as a template (such as RNA virus). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which improves detection throughput and reduces the risk of contamination. Integrating the superior performance of 4G Reverse Transcriptase and hot-start Taq DNA Polymerase, with an optimized buffer system, the detection sensitivity of the 4G One Step RT-PCR Kit can reach 1 pg total RNA and can be amplified Fragments up to 10 kb. The kit is provided in a convenient Master Mix format. The 2 x One-step Mix contains optimized buffer system, dNTP and loading dye; One-step Enzyme Mix contains optimized ratio of 4G Reverse Transcriptase, Murine RNase inhibitor, and hot start Taq DNA polymerase. If the blue dye is added to the 4G One-step RT-PCR Kit, PCR products can be directly loaded for electrophoresis, which greatly saves time and labor.
The PCR reagent concentrations, reaction conditions, and PCR program parameters should be determined experimentally by the investigator.
Features of the 4G One-step RT-PCR Kit:
* High efficiency. Integrating the superior performance of 4G Reverse Transcriptase and hot-start Taq DNA Polymerase, with an optimized buffer system, the detection sensitivity of the 4G One Step RT-PCR Kit can reach 1 pg total RNA and can be amplified Fragments up to 10 kb.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results.
* Reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which improves detection throughput and reduces the risk of contamination.
PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Bst DNA polymerase large fragment.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).
2x PCR master mixes:
* 2x PCR preMix with dye (green, red, blue).
* 2x rapid PCR master mix with a green dye.
* 2x long PCR master mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT supermix for qPCR.
* RT-PCR Kit.
* Dye-based one-step qRT-PCR kits.
* Probe-based one-step qRT-PCR kits.
* Probe-based multiplex one-step qRT-PCR kits.
* SYBR Green qPCR master mixes.
* Probe qPCR master mixes.
PCR components and related products:
* dNTP mix, 10 mM each and dNTP mix, 25 mM each.
* Proteinase K powder and Proteinase K solution.
* Reverse transcriptases and First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* Uracil DNA glycosylases.
* PCR enhancer.
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