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Recombinant Antibody (scFv) Production Services

Chimeric Antibody Production, Recombinant Antibody Expression and Purification
Catalog No. Product Name Size List Price (US$) Quantity
BS079A Molecular Construction of scFv-Fc + 0.03 Liter Transient Production (Template DNA from Clients) 1 package 2200.00
BS079B Molecular Construction of scFv-Fc + 0.03 Liter Transient Production (Variable Regions Synthesized) 1 package 2700.00
BS079C Molecular Construction of scFv-Fc + 0.03 Liter Transient Production (Whole Genes Synthesized) 1 package 3200.00
BS079D 1.5 mg Scale Transient Production of scFv-Fc 0.03 Liter 1000.00
BS079E 14 mg Scale Transient Production of scFv-Fc 0.1 Liter 1500.00
BS079F 75 mg Scale Transient Production of scFv-Fc 0.5 Liter 3750.00
BS079G 250 mg Scale Transient Production of scFv-Fc 2 liter 7000.00
BS079H 1.1 gram Scale Transient Production of scFv-Fc 10 liter 15000.00
Description

Our recombinant antibody (single-chain variable fragment, scFv-Fc) production services include: 1) PCR amplification, or optimization and synthesis of antibody variable regions (VH and VL) or scFv-Fc antibodies for molecular construction of recombinant scFv-Fc antibodies with our proprietary antibody expression vectors; 2) transient production of recombinant antibodies in HEK 293 cells; 3) purification of recombinant antibodies. Made as a single protein, scFv-Fc retains the specificity of the original immunoglobulin, and in certain applications is preferable to whole IgG in construction speed, production yield, and engineering flexibility.

1. Synthesizing antibody variable regions (both VH and VL) or sequences that clients provide:
Including sequence optimization, for free, to meet your goals such as recombinant antibody expression at high and reliable levels.

2. Construction, expression, and purification of scFv-Fc (choice of human or mouse IgG1-Fc backbone; other recombinant IgG-Fc construction is available upon request):
* Connect VH with VL using a short linker peptide of 10-25 amino acids;
* Construct the recombinant scFv-Fc;
* Transiently express the recombinant scFv-Fc protein in HEK 293 cells;
* Purify the recombinant scFv-Fc protein with protein A. Please request if multiple purification methods are required.

The Fc options include but are not limited to:
* Human IgG1-Fc, IgG2-Fc, IgG3-Fc, IgG4-Fc;
* Mouse IgG1-Fc, IgG2a-Fc, IgG2b-Fc, IgG3-Fc;
* Rat IgG1-Fc, IgG2a-Fc, IgG2b-Fc, IgG3-Fc;
* Rabbit IgG-Fc;
* Canine IgG-Fc.

If you provide us the expression vector with your gene already cloned into, we can perform small-scale pilot expression and protein purification by transient transfection into 293 cells to evaluate expression level and yield of the protein of your interest.

Fast turnaround (BS079A, B, and C):
* 1-2 weeks for codon optimization and synthesizing antibody variable regions.
* 1-2 weeks for molecular construction of recombinant scFv-Fc antibodies using E.coli.
* 1-2 weeks for expressing and purifying recombinant scFv-Fc antibodies using mammalian expression systems, CHO or HEK 293 cells.

* BS079D: 0.03 liter transient production, estimated yield 0.5 - 12 mg, average 1.5 mg, 2 weeks;
* BS079E: 0.1 liter transient production, estimated yield 3 - 110 mg, average 14 mg, 2 weeks;
* BS079F: 0.5 liter transient production, estimated yield 12 - 300 mg, average 75 mg, 2 weeks;
* BS079G: 2 liter transient production, estimated yield 50 - 2000 mg, average 250 mg, 3 weeks;
* BS079H: 10 liter transient production, estimated yield 120 - 5000 mg, average 1100 mg, 3 weeks.

Deliverable:
A. Purified scFv-Fc protein (from HEK 293 transient production);
B. Study report.

We also provide the following antibody sequencing and production services:
Clone and sequence hybrimona antibody VH and VL
Construction, expression, and purification of whole IgG antibodies
Generation of stable cell lines expressing antibodies
scFv phage library construction service
ScFv Phage Library Screening Service

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