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Recombinant Antibody (IgG) Production Services

Chimeric Antibody Production, Recombinant Antibody Expression and Purification
Catalog No. Product Name Size List Price (US$) Quantity
BS046A Molecular Construction of Recombinant IgG + 0.03 Liter Transient Production (Template DNA from Clients) 1 package 2200.00
BS046B Molecular Construction of Recombinant IgG + 0.03 Liter Transient Production (Variable Regions Synthesized) 1 package 2700.00
BS046C Molecular Construction of Recombinant IgG + 0.03 Liter Transient Production (Whole Genes Synthesized) 1 package 3200.00
BS046D 2 mg Scale Transient Production of Recombinant IgG 0.03 liter 1000.00
BS046E 10 mg Scale Transient Production of Recombinant IgG 0.1 liter 1500.00
BS046F 50 mg Scale Transient Production of Recombinant IgG 0.5 liter 3750.00
BS046G 200 mg Scale Transient Production of Recombinant IgG 2 liter 7000.00
BS046H 1 gram Scale Transient Production of Recombinant IgG 10 liter 15000.00
Description

Our recombinant antibody production services include: 1) PCR amplification, or optimization and synthesis of antibody variable regions (VH and VL) or whole IgG antibodies for molecular construction of recombinant IgG antibodies with our proprietary antibody expression vectors; 2) transient production of recombinant antibodies in mammalian expression systems, such as CHO and HEK 293 cells; 3) purification of recombinant antibodies. Different antibody constant regions can be expressed with the same antibody variable region to produce chimeric antibodies, which is called isotype switching or class switching. For example, the IgM isotype can be switched to the IgG isotype. The switched chimeric antibody keeps the antigen affinity resulting from the same variable region but interacts with different effector molecules because of the different constant regions.

1. Synthesizing antibody variable regions (VH and VL) or whole IgG antibodies:
Including sequence optimization, for free, to meet goals such as recombinant antibody expression at high and reliable levels. 

2. Construction, expression, and purification of whole IgG antibodies (choice of human or mouse IgG1 backbone; other recombinant IgG construction is available upon request):
* Fuse VH and VL into human or mouse IgG constant region backbone in antibody expression vectors;
* Transiently express the recombinant IgG antibody in HEK 293 cells;
* Purify the recombinant IgG antibody with Protein A. Please request if other purification method or multiple purification methods are required.

The IgG backbone options include but are not limited to:
* Human IgG1, IgG2, IgG3, IgG4;
* Mouse IgG1, IgG2a, IgG2b, IgG3;
* Rat IgG1, IgG2a, IgG2b, IgG3;
* Rabbit IgG;
* Canine IgG.

If you provide us the expression vector with your gene already cloned into, we can perform small-scale (30 ml) pilot expression and protein purification by transfection into HEK 293 cells to evaluate transient expression level and yield of the protein of your interest.

Fast turnaround (BS046A, B, and C):
* 1-2 weeks for codon optimization and synthesizing antibody variable regions.
* 1-2 weeks for molecular construction of recombinant scFv-Fc or IgG antibodies using E.coli.
* 1-2 weeks for expressing and purifying recombinant scFv-Fc or IgG antibodies using HEK 293 cells.

* BS046D: 0.03 liter transient production, estimated yield 0.5 - 10 mg, average 2 mg, 2 weeks;
* BS046E: 0.1 liter transient production, estimated yield 2 - 50 mg, average 10 mg, 2 weeks;
* BS046F: 0.5 liter transient production, estimated yield 10 - 250 mg, average 50 mg, 2 weeks;
* BS046G: 2 liter transient production, estimated yield 40 - 2000 mg, average 200 mg, 3 weeks;
* BS046H: 10 liter transient production, estimated yield 200 - 5000 mg, average 1000 mg, 3 weeks.

Deliverable:
A. Purified antibody (from HEK 293 transient production);
B. Study report.

Related services:
Clone and sequence hybrimona antibody VH and VL
Construction, expression, and purification of single-chain antibodies (scFv-Fc) antibodies
Generation of stable cell lines expressing antibodies

Recombinant IgG Reference Antibodies:
Recombinant Human IgG1 Reference Antibody
Recombinant Human IgG2 Reference Antibody
Recombinant Human IgG4-S228P Reference Antibody
Recombinant Mouse IgG1 Reference Antibody
Recombinant Mouse IgG2a Reference Antibody
Recombinant Mouse IgG2b Reference Antibody

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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