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Rabbit Monoclonal Antibody Production Services

Rabbit Monoclonal Antibody Development, Custom Rabbit Monoclonal Antibody
Catalog No. Product Name Size List Price (US$) Quantity
BS026 Custom Rabbit Monoclonal Antibody Production 1 package Request
Description

Syd Labs has successfully developed rabbit monoclonal antibodies using antigen specific single B cell-based antibody generation technologies. After detection and isolation of antigen specific memory B cells by flow cytometry-based single B cell sorting, the antibody variable regions (VH and VL) from single or clonal B cells are cloned and sequenced. Recombinant rabbit monoclonal antibodies are produced, purified, and tested for various applications, such as ELISA, Western blot, IP, IF, ICC, and IHC. 

The most comprehensive custom rabbit monoclonal antibody production services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications. Syd Labs team has successfully developed a large number of high specificity and high affinity rabbit monoclonal antibodies against a wide range of difficult targets such as small molecules, antibody idiotype, and antigens non-immunogenic in murine, camel or Ilama.

Phase I: Immunization and boosts of 2 rabbits following the standard immunization protocol and ELISA screening of rabbit polyclonal sera. The animal positive respone to the antigen is critical to isolate high specificity and high affinity rabbit monoclonal antibodies.

Phase II: PBMC or splenocyte isolation from selected rabbit(s), detection and isolation of antigen specific memory B cells by flow cytometry-based single B cell sorting, culturing single B cells in individual wells to secret antibodies, high throughput ELISA screening to isolate antigen-positive B cells. Supernatant (culture media) from ELISA-positive clones is sent to clients for evaluation of specific applications.

Phase III: Cloning and sequencing the antibody variable regions (VH and VL) from antigen specific B cells selected by clients, and transient production of recombinant rabbit monoclonal antibodies in HEK 293 cells. Purified rabbit monoclonal antibodies are sent to clients for confirmation and test of specific applications.

You provide: Ten mg of peptide (purity > 85%, length > 12 amino acids) or 4 mg of soluble protein (concentration > 0.4 mg/ml, purity > 90%, molecular weight > 20 kDa) for immunization, and (optional) additional 5 mg for antigen affinity purification.

Deliverable:
* About 80 ul supernatant from ELISA positive cells for evaluation;
* About 100 ug purified rabbit monoclonal antibodies for evaluation;
* Amplified DNA fragments;
* Remaining antigens;
* Study report including ELISA results and antibody VH and VL sequences.

Clients have the choice for licensing antibodies to save cost and even make money.

The prices will be adjusted for the following requirements:

a. Peptide (12-15 amino acids, >85%) design and synthesis +$250 for standard, +$450 for mono-phosphorylated, +$650 for di-phosphorylated, +$700 for mono-methylated (K or R site), +$650 for di-methylated (K or R site), +$700 for tri-methylated (K site), +$400 for acetylated;
b. Rabbit monoclonal antibody production service for modified peptide: +$6,000 for phospho-specific, +$6,000 for methylation-specific, +$6,000 for acetylation-specific;
c. Synthesis of other antigens, +$variable;
d. Conjugation of antigens to protein carriers +$250;
e. Protein: DNA subcloning +$350, bacterial expression and purification +$2,500;
f. Additional boost immunization for higher ELISA titer of the antisera +$150/injection;

Syd Labs also provides other monoclonal antibody development services:

Human and monkey monoclonal antibody development services
Mouse monoclonal antibody development services
Rat monoclonal antibody development services
Camel monoclonal antibody development services

References:

A Robust High Throughput Platform to Generate Functional Recombinant Monoclonal Antibodies Using Rabbit B Cells from Peripheral Blood. Seeber S, Ros F, Thorey I, Tiefenthaler G, Kaluza K, Lifke V, Fischer JA, Klostermann S, Endl J, Kopetzki E, Pashine A, Siewe B, Kaluza B, Platzer J, Offner S. PLoS One. 2014 Feb 4;9(2):e86184.

Generation of Recombinant Monoclonal Antibodies from Immunised Mice and Rabbits via Flow Cytometry and Sorting of Antigen-Specific IgG+ Memory B Cells. Starkie DO, Compson JE, Rapecki S, Lightwood DJ. PLoS One. 2016 Mar 29;11(3):e0152282.

From rabbit antibody repertoires to rabbit monoclonal antibodies. Weber J, Peng H, Rader C. Exp Mol Med. 2017 Mar 24;49(3):e305.

Stable long-term cultures of self-renewing B cells and their applications. Kwakkenbos MJ, van Helden PM, Beaumont T, Spits H. Immunol Rev. 2016 Mar;270(1):65-77.

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