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Protein Expression in Yeast

Catalog No. Product Name Size List Price (US$) Quantity
BS004A Protein Expression in Yeast 1 package Request
BS004B Protein Production from Small to Large Scales 1 package Request
Description

Express your proteins with or without tags in bacterial, yeast, insect, or mammalian cells. We provide free consulting services for selecting expression vectors. If your proteins will be used for antibody development, we provide free consulting services for selecting protein fragments with higher immunogenicity and antigen specificity.

We can optimize stable and transient expression for secreted or intracellular, cytosolic or periplasmic, tagged or untagged proteins. We assess expression levels using the methods of SDS-PAGE, Western, ELISA or functional analysis. We optimize protein expression by modification of the following factors:

a. promoter
b. ribosome binding site
c. vector copy number
d. host cell
e. growth condition including media and additives induction, time, and temperature

Protein purification process can be optimized to meet different requirements:

a. Tag-based affinity purification of recombinant proteins, purity higher than 80%;
b. Purification with ion exchange and/or size exclusion chromatography, purity higher than 90-95%;
c. Customized purification methods for biological activities. If requested, in vitro biological activity will be checked.

Package 1: expression optimization
Package 2: small, middle or large scale expression
Package 3: purification optimization
Package 4: expression optimization + purification optimization
Package 5: small, middle or large scale purification
Package 6: DNA subcloning + expression optimization + purification optimization + purification
Package 7: DNA subcloning + expression optimization + purification optimization + purification (guaranteed)
Package 8: gene synthesis + DNA subcloning + expression optimization + purification optimization + purification
Package 9: gene synthesis + DNA subcloning + expression optimization + purification optimization + purification (guaranteed)

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