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Home > Services > Bioanalytical Services > Protein Analysis Services
Analysis of proteins by LC-MS/MS and HPLC.
    14 Protein Analysis Services
  • Denaturation and reduction followed by LC-MS (liquid chromatography-mass spectrometry) analysis.

    Fast turnaround time: 1- 2 weeks.

    Sample requirement: >100 ug at 1 mg/ml.

  • Peptide mapping by LC-MS/MS can be used to determine detailed information about the peptide's sequence and post-translational modifications.
     

  • De novo antibody sequencing to obtain the intact amino acid sequence of variable and/or constant regions of monoclonal antibodies by LC-MS/MS.

    Sample requirement: 3-5 mg at 1 mg/ml preferred (minimum: 2 mg at 1 mg/mL).

    Fast turnaround time: 2-3 months per antibody, but may require more time depending on the complexity of analysis.

  • Capillary electrophoresis (CE) analysis using Caliper LC90.

    Sample requirement: 50-100 ug.

    Fast turnaround time: 1 - 2 weeks.

  • Capillary isoelectric focusing is an analytical technique for characterization of protein mixtures and determination of protein isoelectric points.

    Sample requirement: 50-100 ug.

    Fast turnaround time: 1 - 2 weeks.

  • Sample requirement: 50-100 ug.

    Fast turnaround time: 1 - 2 weeks.

  • Ion-Exchange HPLC (IEX-HPLC) for separation of samples based on differences in their surface charge.

    Sample requirement: 100-200 ug.

    Fast Turnaround time: 1 - 2 weeks.
  • Normal-phase chromatography (NP-HPLC) is an analytical technique that is used to separate molecules on the basis of differences in the strength of their interaction with a polar stationary phase.

    Sample requirement: 100-200 ug.

    Fast turnaround time: 2 - 3 weeks.
  • Reversed-phase chromatography (RP-HPLC) is an analytical technique that separates molecules on the basis of differences in their hydrophobicity.

    Sample requirement: 50-100 ug.

    Fast turnaround time: 1 - 2 weeks.
  • The antibody variable regions of both heavy chain (VH) and light chain (VL) are analyzed for liabilities including, but not limited to N-linked glycosylation, deamidation, isomerization, pyroglutamate formation, and oxidation.

    Fast turnaround time: 1 week per antibody, but may require more time depending on the complexity of analysis.

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