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Home > Molecular Biology > PCR > PCR Master Mixes
Convenient premix reagents needed for various PCR, including RT-PCR and Real-Time PCR.
    5 PCR Master Mixes Products
  • MB040-HY2: 2x HY PCR Master Mix, with blue dye

    * The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
    * The high yield PCR Master Mix has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates.
    * The tracking dye and precipitant have been added into the PCR PreMix so that the PCR product can be directly loaded for electrophoresis.

    Features of the blend of Taq enzyme and a proofreading enzyme contained in the high yield PCR master mix:
    * Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
    * High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
    * Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
    * Proofreading with 5′ exonuclease activity.

  • MB067-EQ2G / MB067-EQ2B / MB067-EQ2R: 2x PCR PreMix, with dye (green, blue, or red)

    * The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
    * The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.

    Features of the Taq DNA polymerases contained in the PCR master mix:
    * High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
    * High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
    * Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
    * Terminal transferase activity of adding a single nucleotide (adenosine) at 3'-end of the extension product, facilitating TA cloning of PCR products.

  • MB073-PCR: 2x Long Taq PCR MasterMix (With Dye)

    For reaction set-up, all you have to do is to add templates, specific primers, and water.

  • MB103-11: Cell and Tissue Direct PCR Kit (rapid genomic DNA extraction coupled with PCR)

    Solution based single tube protocol for rapid genomic DNA extraction;
    Extract genomic DNA from animal cells and tissue samples such as mouse tail and cultured cells in <10 minutes;
    No toxic chemicals, DNA precipitation and column purification, or proteinase digestion;
    Time saving, convenient, consistent, and minimal risk of contamination and pipetting errors;
    The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.

  • MB103-21: Blood Direct PCR Kit (rapid genomic DNA extraction coupled with PCR)

    Solution based single tube protocol for rapid genomic DNA extraction;
    Extract genomic DNA from fresh or anti-coagulated blood in <10 minutes;
    No toxic chemicals, DNA precipitation and column purification, or proteinase digestion;
    Time saving, convenient, consistent, and minimal risk of contamination and pipetting errors;
    The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.

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