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N-terminal Protein Sequencing Service

N-terminal Edman Sequencing of Proteins/Peptides
Catalog No. Product Name Size List Price (US$) Quantity
BS072A N-terminal Protein Sequencing Service (5 residues) 1 sample 350.00
BS072B N-terminal Protein Sequencing Service (10 residues) 1 sample 450.00
BS072B N-terminal Protein Sequencing Service (15 residues) 1 sample 550.00
Description

N-terminal Edman sequencing of proteins or peptides can be used to determine the N-terminal amino acid residues of purified protein samples in addition to cleavage sites, and to identify an unknown protein or confirm the identity of a known protein, or to design oligonucleotide probes. Advantages of N-terminal Edman sequencing include high sensitivity, even with picomoles of protein. Proteins can be sequenced in solution, gels or on PVDF blots.

General procedure of N-terminal protein sequencing:

N-terminal Edman sequencing of proteins will be performed with five to twenty residues (degradation steps), depending on information needed as follows:

A. 5 residues are recommended for determination of a cleavage site in a known protein.
B. 5-10 residues are recommended to confirm the identity of an isolated known protein.
C. 10-20 residues are recommended to design oligonucleotide probes.
D. 15 or more residues are recommended to identify an unknown protein.

Sample requirements:

Sample size: 50 ug protein;
Formulation: freeze-dried or dissolved in 1X PBS or equivalent buffers;
Purity: > 95%.

Deliverable:

A. Report with N-terminal amino acid sequences of purified protein samples.
Note: if N-terminal of heavy chain is blocked, N-terminal sequence may not be obtained.

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