Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
The prices described above are based on analysis using our standardized methods, proven to work for the majority of antibodies and proteins. Method development options may be necessary for atypical proteins with unique properties.
A setup fee may apply depending on the order volume.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
N-terminal Edman sequencing of proteins or peptides can be used to determine the N-terminal amino acid residues of purified protein samples in addition to cleavage sites, and to identify an unknown protein or confirm the identity of a known protein, or to design oligonucleotide probes. Advantages of N-terminal Edman sequencing include high sensitivity, even with picomoles of protein. Proteins can be sequenced in solution, gels or on PVDF blots.
General procedure of N-terminal protein sequencing:
N-terminal Edman sequencing of proteins will be performed with five to twenty residues (degradation steps), depending on information needed as follows:
A. 5 residues are recommended for determination of a cleavage site in a known protein.
B. 5-10 residues are recommended to confirm the identity of an isolated known protein.
C. 10-20 residues are recommended to design oligonucleotide probes.
D. 15 or more residues are recommended to identify an unknown protein.
Sample requirements:
Sample size: 50 ug protein;
Formulation: freeze-dried or dissolved in 1X PBS or equivalent buffers;
Purity: > 95%.
Deliverable:
A. Report with N-terminal amino acid sequences of purified protein samples.
Note: if N-terminal of heavy chain is blocked, N-terminal sequence may not be obtained.
Copyright © 2009-2022 sydlabs.com. All rights reserved.
Use of this website means that you have read, understood, and accepted the Syd Labs Privacy Policy and Terms & Conditions.