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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal LIF Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIFs exhibit a 78% identity in their amino acid sequences.
BP000092-GPF-001: Recombinant Murine Leukemia Inhibitory Factor Protein, GST tag
Source: E. coli-derived.
mLIF supplement is assessed both on mESCs and on murine M1 myeloid leukemic cells. A standard of 50 units is defined as the concentration of mLIF supplement in 1.0 ml of tissue culture medium that induces the differentiation of 50% of M1 colonies (Metcalf, 1988).
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/ml.
Murine myeloid leukemic M1 Assay: Specific Activity >108 units/mg.
Purity: > 95% by SDS-PAGE.
Formulation: Dissolved in solution with PBS, pH 7.3 and 0.1% BSA (w/v). BSA serves as a protein carrier for stability.
Concentration: 107 units/ml.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20°C to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20°C to -70°C under sterile conditions after reconstitution.
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